Effect of bovine seminal ribonuclease (BS-RNase) on pigs bone marrow cells

• Published in: Archiv für Tierzucht Vol. 44; no. 1; pp. 53 - 64
• Main Authors: Matoušek, J., Hruban, V., Hradecky, J., Hrubá, A., Soućek, J.
• Format: Journal Article
• Language: English
• Published: 10.10.2001
• ISSN: 2363-9822; 2363-9822
• DOI: 10.5194/aab-44-53-2001

The effect of bovine seminal ribonuclease (BS RNase) on bone marrow cells in miniature pigs was studied. BS RNase at the concentration 20 and l00 ug/ml preincubated 1 h with bone marrow cells from normal miniature pigs did not influence the formation of CFU-GM and BFU-E colonies cultivated in methylcellulose medium. The bone marrow cells kept in 4 °C survived better in the presence of BS RNase than control cells cocultivated with bovine serum albumin (BSA). There was proved that pig bone marrow cells are able to bind the BS RNase on their surface without any morphological damage. For the experimental bone marrow transplantation in miniature pigs the whole body irradiation of 8G with a cobalt source was used. Two recipients from seven accepted semi-incompatible bone marrow cells preincubated 2 h with BS RNase (10 mg/5 x l08 cells). No recipient from eight transplanted control animals accepted semi-incompatible bone marrow cells preincubated with BSA.