Age related changes in hemopoietic capacity of bone marrow cells

The effect of aging on the hemopoietic capacity of bone marrow (BM) cells was investigated. No difference was found in the incidence of colony forming units-spleen (CFU-S) and granulocyte-macrophage colony forming cells (GM-CFC) present in the BM of young (2–4 months) or old (24–30 months) mice. How...

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Published inMechanisms of ageing and development Vol. 48; no. 1; pp. 91 - 99
Main Authors Sharp, A., Zipori, D., Toledo, J., Tal, S., Resnitzky, P., Globerson, A.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 01.04.1989
Elsevier Science
Subjects
Aging
Aging - physiology
Animals
Biological and medical sciences
Bone marrow
Bone Marrow - immunology
Bone Marrow - physiology
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Cells, Cultured
CFU-S
Fundamental and applied biological sciences. Psychology
GM-CFC
Granulocytes - physiology
Hematopoiesis
Hematopoietic Stem Cells - physiology
Macrophages - physiology
Male
Mice
Molecular and cellular biology
Spleen - physiology
Thymus Gland - physiology
Aging
Bone marrow
CFU-S
GM-CFC
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Summary:The effect of aging on the hemopoietic capacity of bone marrow (BM) cells was investigated. No difference was found in the incidence of colony forming units-spleen (CFU-S) and granulocyte-macrophage colony forming cells (GM-CFC) present in the BM of young (2–4 months) or old (24–30 months) mice. However, increased proliferations (× 3) of the old BM cells was observed when cultured in the presence of L-cell conditioned medium. The cells were also cultivated over an adherent layer of a BM stroma cell line (14F1.1, endothelial-adipocytes) and the non-adherent cells removed and tested weekly. Cells orginating from the old BM proliferated to a greater extent and produced more GM-CFC than those from the young. Differentiation into T-cells after colonizing fetal thymus explants was also measured and found to be reduced in both groups, though to a greater extent in the old. Thus, under the present experimental conditions, myeloid progenitors in the old BM manifest a more pronounced self renewal and differentiation capacity than the young while for the T-cell pregenitors the situation is reversed.
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ISSN:0047-6374
1872-6216
DOI:10.1016/0047-6374(89)90029-8