Investigation of antidepressant-like effect of dipyrone using chronic unpredictable mild stress model

• Published in: Proceedings for Annual Meeting of The Japanese Pharmacological Society Vol. WCP2018; p. PO3-1-28
• Main Authors: Aksu, Fazilet, Kiroglu, Olcay, Demirkol, Kubra, Berktas, Fatih, Yegani, Arash A, Maytalman, Erkan, Unverdi, Mahmut
• Format: Journal Article
• Language: English
• Published: Japanese Pharmacological Society 2018
• Subjects: Antidepressant-like effect; Dipyrone; UCMS
• ISSN: 2435-4953; 2435-4953
• DOI: 10.1254/jpssuppl.WCP2018.0_PO3-1-28

Background: An analgesic-antipyretic drug dipyrone shows its effects via central prostaglandin system, opiatergic and cannabinoid systems, cytokine levels, TRP channels and descending serotonergic-noradrenergic pathways. The functional roles of all these mechanisms have been established also in mood disorders. It is known that pain and depression are frequently seen together. In the light of these information we aimed to investigate whether dipyrone has an antidepressant-like effect. Methods: Swiss albino mice were used in all experiments. Depression-like effects were investigated in an unpredictable chronic mild stress (UCMS) model in both male and female mice. Cage changes, light-dark cycle reversal, cage tilting, wet floor, empty cage, foreign material on the floor and predator sounds were used different times to induce light stress at mice for six weeks. Dipyrone was administered intraperitoneal beginning from the third week. Splash, rota-rod (RR) and forced swimming (FST) tests were performed at the seventh week as behavioral tests to evaluate the antidepressant-like effects of dipyrone. Coat state score (CSS) and weights of animals were recorded at the beginning of each week. Results were analyzed using one or two-way ANOVA followed by the Bonferonni post hoc test.Results: Weight of UCMS-exposed mice did not change compared with controls; however, significant changes were observed in CSS in both sexes of stressed mice (p<0.05). RR latency decreased and immobility time enhanced in FST test in both sexes of stressed mice (p<0.05). Grooming behavior was not different between the groups in female mice, but different in male mice in the splash test. Dipyrone did not produce a significant change in CSS in the UCMS-exposed group but reversed the latency time and immobility time to normal values in both sexes of mice and augmented the number of grooming behavior only in stressed male mice.Conclusions: These results indicate that dipyrone produce antidepressant-like effects to some symptoms of UCMS according to gender.