Effect of the silencing of TNF alpha and glycine receptors on the secretion of pro-inflammatory cytokines in 3T3-L1 preadipocytes

• Published in: Proceedings for Annual Meeting of The Japanese Pharmacological Society Vol. WCP2018; p. PO3-6-26
• Main Authors: Romero-Nava, Rodrigo, Contreras-Nunez, Erika, Blancas-Flores, Gerardo, Almanza-Perez, Julio C., Giacoman-Martinez, Abraham, Roman-Ramos, Ruben, Huang, Fengyang, Alarcon-Aguilar, Francisco J.
• Format: Journal Article
• Language: English
• Published: Japanese Pharmacological Society 2018
• Subjects: Glycine receptor; Inflammation; TNF alpha receptor
• ISSN: 2435-4953; 2435-4953
• DOI: 10.1254/jpssuppl.WCP2018.0_PO3-6-26

Obesity has been related to a process of chronic inflammation, which is characterized by an increase in pro-inflammatory cytokine levels. It has been reported that glycine decreases the secretion of TNF alpha and IL-6 in adipocytes, although the mechanisms have not yet been clarified. The purpose of this study was to determine the mechanism by which glycine reduces the secretion of TNF alpha and IL-6 using siRNA from the TNF alpha and glycine receptors.METHODS: Several types of glycine receptors were characterized by RT-PCR. 3T3-L1 cells were transfected with siRNA for glycine receptor; three days later, the cells were ready for the experiments. 3T3-L1 cells was treated with Glycine 10 mM. Total RNA was extracted from the cells for the determination of TNF alpha and IL-6 expression by RT-PCR; in addition, the concentration of these cytokines in the medium was quantified by ELISA. RESULTS: Glycine decreased the production and release of TNF alpha and IL-6, whereas treatment with siRNA for the TNF alpha and glycine receptors produced increases in the secretion of the levels of these cytokines.CONCLUSION: These findings give clues about the signaling mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in obesity.