Expression of cloned beta-endorphin gene sequences by Escherichia coli

DNA coding for the opiate peptide beta-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid beta-galactosidase/beta-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active beta-endorphin.

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Bibliographic Details
Published inNature (London) Vol. 285; no. 5765; p. 456
Main Authors Shine, J, Fettes, I, Lan, N C, Roberts, J L, Baxter, J D
Format Journal Article
LanguageEnglish
Published England 12.06.1980
Subjects
Animals
beta-Galactosidase - genetics
Cloning, Molecular - methods
DNA, Recombinant
Endorphins - genetics
Endorphins - immunology
Escherichia coli - genetics
Genes
Lac Operon
Mice
Plasmids
Receptors, Opioid - metabolism
Structure-Activity Relationship
Trypsin - metabolism
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Summary:DNA coding for the opiate peptide beta-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid beta-galactosidase/beta-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active beta-endorphin.
ISSN:0028-0836
DOI:10.1038/285456a0