Identity of a differentiation inhibiting factor for mouse myeloid leukemia cells with NM23/nucleoside diphosphate kinase

• Published in: Biochemical and biophysical research communications Vol. 182; no. 3; pp. 987 - 994
• Main Authors: Okabe-Kado, Junko, Kasukabe, Takashi, Honma, Yoshio, Hayashi, Moriaki, Henzel, William J., Hozumi, Motoo
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 14.02.1992; Elsevier
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; Cell Differentiation - drug effects; Cell Line; Clone Cells; Dexamethasone; Electrophoresis, Polyacrylamide Gel; General aspects (metabolism, cell proliferation, established cell line...); Growth Inhibitors - physiology; Humans; Interleukin-6; Leukemia Inhibitory Factor; Leukemia, Experimental; Lymphokines - physiology; Medical sciences; Mice; Molecular Sequence Data; Nucleoside-Diphosphate Kinase - isolation & purification; Nucleoside-Diphosphate Kinase - physiology; Peptide Fragments - isolation & purification; Phagocytosis; Sequence Homology, Nucleic Acid; Tumor cell; Tumors; Purification; Enzyme; Rodentia; Malignant hemopathy; Malignant tumor; Metastasis; Cell differentiation; In vitro; Nucleoside diphosphate kinase; Biological activity; Vertebrata; Mammalia; Mouse; Animal; Chronic myelocytic leukemia; Inhibition; Tumor cell
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(92)91829-F

Mouse myeloid leukemic line M1 cells can be induced to differentiate into the monocyte/macrophage pathway by various inducers. The induction of differentiation of M1 cells can be inhibited by protein inhibitors termed differentiation inhibiting factors (I-factors) in a cell lysate and conditioned medium of differentiation resistant M1 cells. Production of the I-factor activity in resistant M1 cells is well associated with development of resistance of M1 cells to differentiation inducers. We have now purified one of the I-factors from conditioned medium of differentiation resistant M1 cells. The purified I-factor has a relative molecular mass of approximately 16000–17000 Da (16K I-factor). The amino acid sequence of all fragments of the 16K I-factor we have found are identical with Nm23/nucleoside diphosphate kinase (EC2.7.4.6) protein involved in tumor metastasis. The findings indicate that the I-factor, a candidate suppressor protein for differentiation of leukemic cells, is Nm23/nucleoside diphosphate kinase protein.