Glutaraldehyde stabilisation of antibody-linked erythrocytes for use in reverse passive and related haemagglutination assays

A simple method for stabilising antibody-linked red blood cells by the addition of low concentrations of glutaraldehyde is described. Fresh and stabilised reagent-linked cells were shown to compare favourably in reverse passive haemagglutination for the measurement of human immunoglobulin isotypes,...

Full description

Saved in:
Bibliographic Details
Published inJournal of immunological methods Vol. 64; no. 1-2; p. 7
Main Authors Cranage, M P, Gurner, B W, Coombs, R R
Format Journal Article
LanguageEnglish
Published Netherlands 11.11.1983
Subjects
Animals
Antibodies
Antibodies, Monoclonal
Brucellosis - diagnosis
Cattle
Drug Stability
Erythrocytes - immunology
Glutaral
Hemagglutination Tests - methods
Sheep
Simplexvirus - immunology
Simplexvirus - isolation & purification
Online AccessGet more information

Cover

More Information
Summary:A simple method for stabilising antibody-linked red blood cells by the addition of low concentrations of glutaraldehyde is described. Fresh and stabilised reagent-linked cells were shown to compare favourably in reverse passive haemagglutination for the measurement of human immunoglobulin isotypes, G, A and M and for the detection of respiratory syncytial and herpes simplex viruses. Stabilised cells were also used to detect antibodies to bacteria and to a soluble antigen adsorbed to a solid phase by mixed haemagglutination reactions.
ISSN:0022-1759
DOI:10.1016/0022-1759(83)90378-2