The Streptomyces aureofaciens homologue of the whiG gene encoding a putative sigma factor essential for sporulation

• Published in: Gene Vol. 143; no. 1; pp. 101 - 103
• Main Authors: Kormanec, Ján, Potúčková, Laura, Řezuchová, Bronislava
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 27.05.1994; Amsterdam Elsevier; New York, NY
• Subjects: Amino Acid Sequence; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Base Sequence; Biological and medical sciences; Cell Differentiation; Cloning, Molecular; development; DNA, Bacterial - genetics; DNA-Binding Proteins; Fundamental and applied biological sciences. Psychology; Genes, Bacterial - genetics; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutagenesis, Insertional; Open Reading Frames; Recombinant DNA; RNA polymerase; Sequence Alignment; sequence comparison; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Sigma Factor - chemistry; Sigma Factor - genetics; Spores, Bacterial - growth & development; Streptomyces - genetics; Streptomyces aureofaciens - genetics; Streptomyces coelicolor; Streptoverticillium griseocarneum; aa; St; development; B; ORF; RBS; SDS; Sv; Streptomyces coelicolor; E; nt; Recombinant DNA; RNA polymerase; bp; P; R; S; PolIk; Th; dCTP; sequence comparison; oligo; Streptoverticillium griseocarneum; wt; Actinomycetales; Streptomycetaceae; Nucleotide sequence; Sporulation; Initiation factor σ; Bacteria; Chromosome DNA; Homology; Actinomycetes; Mutation; Streptomyces aureofaciens
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(94)90612-2

A putative σ factor-encoding gene, rpoZ, was cloned from Streptomyces aureofaciens. Its deduced protein product showed high similarity to the putative σ factor encoded by the Streptomyces coelicolor whiG gene. Disruption of rpoZ blocked differentiation at a stage between the formation of aerial mycelium and the development of mature spores.