Monoclonal antibodies to novel erythroid differentiation antigens reveal specific effects of oncogenes on the leukaemic cell phenotype

• Published in: Leukemia research Vol. 10; no. 3; pp. 257 - 272
• Main Authors: Schmidt, Jerzy A., Marshall, John, Hayman, Michael J., Doderlein, Gabriele, Beug, Hartmut
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1986; Elsevier Science
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - isolation & purification; Antigens, Surface - analysis; Biological and medical sciences; Bone Marrow - immunology; Carcinogenesis, carcinogens and anticarcinogens; Cell Differentiation; Cell Division; Cell Transformation, Viral; Chickens; Erythrocytes - immunology; Erythroid differentiation; Hematopoietic Stem Cells - cytology; Leukemia - immunology; Leukemia - pathology; Leukemia, Erythroblastic, Acute - immunology; Macrophages - immunology; Medical sciences; Mice; Mice, Inbred BALB C; monoclonal antibodies; Oncogenes; Phenotype; Rabbits; Tumors; Viruses; CFU-E, colony forming unit-erythroid; FCS, foetal calf serum; Hepes, N-2-hydroxyethylpiperazine- N′-2-ethane sulphonic acid; Erythroid differentiation; HAT, hypoxanthine aminopterin thymidine; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis; oncogenes; AEV, Avain erythroblastosis virus; cMGF, chicken myelomonocytic growth factor; monoclonal antibodies; Erythropoiesis; Erythroid cell; V-Onc gene; Biological marker; Monoclonal antibody; Cell surface; Leukosis; Aves; Tumor cell; Oncovirinae; Retroviridae; Malignant hemopathy; Cell differentiation; Gene expression; Infection; Virus; Antigen; Vertebrata; Phenotype; Alloantigen; Type C oncovirus; Animal; Viral disease; Avian leukosis virus; Chicken; Comparative study
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/0145-2126(86)90023-8

A panel of new monoclonal antibodies to antigens on the surface of chick erythroid progenitor cells is described. These are characterised with respect to their binding to different classes of normal haemopoietic cells of both the erythroid and myeloid lineages. Using these antibodies, we have examined the phenotype of avian leukaemic cells transformed by retroviruses carrying defined oncogenes. Our data show that these cells, although similar to the normal haematopoietic precursor from which they are derived, aberrantly express certain markers in an oncogene specific manner.