Differential light-scattering spectroscopy: a new approach to studying of colloidal gold nanosensors

Addition of complementary components to a bioconjugate probe results in aggregation of nanoparticles that can be monitored by extinction spectra. This is the core idea of the well-known sol particle immunoassay (SPIA) introduced by Leuvering et al. (J. Immunoassay 1 (1980) 77). Here, we describe a n...

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Published inJournal of quantitative spectroscopy & radiative transfer Vol. 89; no. 1-4; pp. 133 - 142
Main Authors Khlebtsov, N.G., Bogatyrev, V.A., Melnikov, A.G., Dykman, L.A., Khlebtsov, B.N., Krasnov, Ya.M.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 15.11.2004
Subjects
Aggregation
Bioconjugates
Gold nanoparticles
Plasmon resonance
Resonance light scattering
T-matrix method
Aggregation
T-matrix method
Bioconjugates
Plasmon resonance
Gold nanoparticles
Resonance light scattering
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Summary:Addition of complementary components to a bioconjugate probe results in aggregation of nanoparticles that can be monitored by extinction spectra. This is the core idea of the well-known sol particle immunoassay (SPIA) introduced by Leuvering et al. (J. Immunoassay 1 (1980) 77). Here, we describe a new approach to study the biospecific interactions in systems of colloidal gold bioconjugates. The method is based on measuring static light-scattering spectra (at 90∘) within a wavelength range of 350–800nm. To this end, we have developed a special attachment to the Specord M-40 spectrophotometer and a corresponding measurement procedure called by us “differential light-scattering spectroscopy” (DLSS). The DLSS technique has been compared with conventional spectrophotometry as applied to colloidal gold conjugates with various biopolymers. Our theoretical simulations and experiments with gold particles of various sizes showed a higher potential sensitivity of the method proposed as compared with conventional extinction spectroscopy. It is expected that DLSS can be used to develop an analytical biospecific test for various biopolymers.
ISSN:0022-4073
1879-1352
DOI:10.1016/j.jqsrt.2004.05.017