In vitro infection of human B lymphocytes with adult T-cell leukemia virus

• Published in: Cancer letters Vol. 22; no. 1; pp. 11 - 21
• Main Authors: Okada, Morihisa, Koyanagi, Yoshio, Kobayashi, Nobuyuki, Tanaka, Yuetsu, Nakai, Masuyo, Sano, Kouichi, Takeuchi, Kaoru, Hinuma, Yorio, Hatanaka, Masakazu, Yamamoto, Naoki
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.02.1984; Elsevier
• Subjects: AIDS/HIV; Antigens, Viral - analysis; B-Lymphocytes - immunology; Biological and medical sciences; Cell Transformation, Viral; Clone Cells; Deltaretrovirus - immunology; Deltaretrovirus - pathogenicity; Deltaretrovirus - ultrastructure; Hematologic and hematopoietic diseases; Herpesvirus 4, Human - immunology; Herpesvirus 4, Human - ultrastructure; Humans; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Microscopy, Electron; RNA, Viral - isolation & purification; B-Lymphocyte; Epstein Barr virus; Leukemia; Cellulose hydrate; T-Lymphocyte
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/0304-3835(84)90038-7

Experimental transmission of adult T-cell leukemia (ATL) virus (ATLV) into human B lymphocytes was attempted. Cocultivation of B-cell rich fraction of peripheral blood from a healthy adult with X-ray irradiated ATLV producer MT-2 cells resulted in the establishment of OKA(B) cell line co-infected with both Epstein-Barr virus (EBV) and ATLV. OKA(B) cells and its subclones contained: (1) B cell markers exclusively; (2) both EBV-specific antigen, EBNA and ATLV-specific antigen, ATLA detected by immunofluorescence test; (3) ATLV-specific polypeptides, p24 and p19; (4) ATLV-specific mRNA in ATLA-positive clones; (5) ATLV and EBV particles detectable by electron microscopy. These data clearly show that human B lymphocytes are susceptible to ATLV infection.