Genetic Polymorphism and Variability of the Anacamptis morio s.l. (Orchidaceae Juss.) Population in Ukraine

• Published in: Cytology and genetics Vol. 55; no. 4; pp. 299 - 308
• Main Authors: Gaponenko, M. B., Blum, O. B., Kashevarov, G. P.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2021; Springer Nature B.V
• Subjects: Anacamptis morio; Biomedical and Life Sciences; Biomedicine; Black Sea; DNA; DNA sequencing; flora; Gene flow; Gene polymorphism; Genetic analysis; Genetic distance; Genetic diversity; genetic polymorphism; genetic similarity; genetic variation; Human Genetics; Polymorphism; Population differentiation; Population genetics; Population studies; Random amplified polymorphic DNA; Ukraine; Crimea; Ukraine; Black Sea; RAPD; ISSR markers; rare plants; Ukraine; genetic polymorphism; PCR analysis; sequencing
• ISSN: 0095-4527; 1934-9440
• DOI: 10.3103/S0095452721040046

The results of the population genetic and taxonomic analysis of the critical species of Orchids, viz. Anacamptis morio s.l. in the flora of Ukraine are presented. The genetic analysis was carried out using PCR analysis (RAPD-, ISSR amplification) and DNA sequencing ( matK , atpF-atpH , rbcL ) and genetic differentiation of populations of these plants from Transcarpathian Ukraine (Zakarpattia), Ukrainian Polissia, and Crimea. A significant level of genetic difference between the Crimean populations and populations from Zakarpattia and Ukrainian Polissia, as well as a high level of genetic similarity between all of the studied populations of A. morio subsp. caucasica from Crimea, was established. The highest level of genetic polymorphism of the studied populations was detected by the RAPD-markers OPA-11, OPP-10, OPA-18, and UBC-297 as well as the ISSR-marker (AG)8TC. The main indices of genetic polymorphism of the five A. morio populations were as follows: the proportion of polymorphic loci (P) was 40.0–74.8%, the effective number of alleles ( n e ) was 1.276–1.479, the genetic diversity (h) was 0.156–0.276, the Shannon information index (I) was 0.229–0.408, and Nei’s genetic distances between populations (D N ) was 0.047–0.325. For a more complete genetic characterization of populations, the following characteristics were also determined: expected total genetic diversity (H t ), intrapopulation genetic diversity (H S ), population differentiation coefficient (G st ), and the gene flow value (N m ). Based on the results of molecular genetic studies and the analysis of morphological and biological data in the flora of Ukraine, it is proposed to delimitate the taxa as follows: A. morio subsp. morio (L.) R.M. Bateman, Pridgeon et M.W. Chase, which grows in the Ukrainian Carpathians and the Polissia as well as A. morio subsp. caucasica (K. Koch) H. Kretzschmar, Eccarius et H. Dietr. distributed in the Crimea and the Northern Black Sea region on the territory of Ukraine.