More detailed characterization of some of the HL60 karyotypic features by fluorescence in situ hybridization

We performed a focused chromosome analysis on the HL60 cell line by multicolor fluorescence in situ hybridization (FISH), using probes for the unequivocal identification of specific chromosome regions and subregions. The purpose of this karyotypic re-evaluation was to confirm and to characterize in...

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Bibliographic Details
Published inCancer genetics and cytogenetics Vol. 87; no. 2; pp. 103 - 106
Main Authors Volpi, Emanuela V., Vatcheva, R., Labella, T., Gan, S.U.
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.04.1996
Elsevier Science
Subjects
Biological and medical sciences
Chromosome Aberrations
Chromosomes, Artificial, Yeast
Chromosomes, Human, Pair 17
Hematologic and hematopoietic diseases
HL-60 Cells
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia, Myeloid, Acute - genetics
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Human
Molecular hybridization
Cell line
In situ
Leukemia
Cytogenetics
Fluorescence
Malignant hemopathy
Molecular biology
Karyotype
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Summary:We performed a focused chromosome analysis on the HL60 cell line by multicolor fluorescence in situ hybridization (FISH), using probes for the unequivocal identification of specific chromosome regions and subregions. The purpose of this karyotypic re-evaluation was to confirm and to characterize in more detail chromosome rearrangements already identified by means of classic cytogenetic approaches and recurrently detected from the initial establishment of the cell line. The observations reported may help reassess the potential of the HL60 cell line in understanding the molecular events underlying the non-random karyotype alterations associated with acute myeloid leukemias (AML).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0165-4608
1873-4456
DOI:10.1016/0165-4608(95)00214-6