Sensitive determination of glycerol by derivatization using a HPLC-DAD method in biodiesel samples
Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through...
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| Published in | Analytical methods Vol. 7; no. 18; pp. 7805 - 7810 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
01.01.2015
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product
i.e.
glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 μg mL
−1
and 0.76 μg mL
−1
, respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. |
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| AbstractList | Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product i.e.glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 mu g mL super(-1) and 0.76 mu g mL super(-1), respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product i.e. glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 μg mL −1 and 0.76 μg mL −1 , respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. |
| Author | Khan, Ijaz Musharraf, Syed Ghulam Ahmed, Muhammad Arif Hashim, Jamshed |
| Author_xml | – sequence: 1 givenname: Muhammad Arif surname: Ahmed fullname: Ahmed, Muhammad Arif organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan – sequence: 2 givenname: Ijaz surname: Khan fullname: Khan, Ijaz organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan – sequence: 3 givenname: Jamshed surname: Hashim fullname: Hashim, Jamshed organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan – sequence: 4 givenname: Syed Ghulam surname: Musharraf fullname: Musharraf, Syed Ghulam organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan |
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| SubjectTerms | Arrays Biodiesel Byproducts Chlorides Copper chlorides Diodes Glycerols Mathematical analysis Transesterification |
| Title | Sensitive determination of glycerol by derivatization using a HPLC-DAD method in biodiesel samples |
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