Sensitive determination of glycerol by derivatization using a HPLC-DAD method in biodiesel samples
Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through...
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Published in | Analytical methods Vol. 7; no. 18; pp. 7805 - 7810 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2015
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Abstract | Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product
i.e.
glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 μg mL
−1
and 0.76 μg mL
−1
, respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. |
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AbstractList | Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product i.e.glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 mu g mL super(-1) and 0.76 mu g mL super(-1), respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product i.e. glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 μg mL −1 and 0.76 μg mL −1 , respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. |
Author | Khan, Ijaz Musharraf, Syed Ghulam Ahmed, Muhammad Arif Hashim, Jamshed |
Author_xml | – sequence: 1 givenname: Muhammad Arif surname: Ahmed fullname: Ahmed, Muhammad Arif organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan – sequence: 2 givenname: Ijaz surname: Khan fullname: Khan, Ijaz organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan – sequence: 3 givenname: Jamshed surname: Hashim fullname: Hashim, Jamshed organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan – sequence: 4 givenname: Syed Ghulam surname: Musharraf fullname: Musharraf, Syed Ghulam organization: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan |
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CitedBy_id | crossref_primary_10_1016_j_talanta_2016_03_033 crossref_primary_10_1134_S1061934820020112 crossref_primary_10_1039_D4RA02985B crossref_primary_10_1021_acs_analchem_1c02463 crossref_primary_10_1007_s10337_018_3681_3 crossref_primary_10_1016_j_chroma_2015_10_036 crossref_primary_10_1007_s00216_024_05316_7 |
Cites_doi | 10.1016/j.microc.2012.04.011 10.1016/j.talanta.2011.10.055 10.1016/j.talanta.2010.09.061 10.1002/ejlt.200401054 10.1016/j.chroma.2007.04.063 10.1016/j.jchromb.2014.02.054 10.1002/ejlt.200600004 10.1016/j.fuel.2008.04.033 10.1021/jo00074a006 10.1002/anie.200604977 10.1016/j.jchromb.2009.09.015 10.1007/s00216-011-5058-4 10.1016/0021-9673(94)00867-9 10.1016/j.talanta.2009.03.013 10.1016/j.fuel.2011.06.071 10.1016/j.fuel.2010.07.046 |
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References | Lima (C5AY01761K-(cit13)/*[position()=1]) 2012; 89 Stelmachowski (C5AY01761K-(cit1)/*[position()=1]) 2011; 18 Plank (C5AY01761K-(cit9)/*[position()=1]) 1995; 697 Silva (C5AY01761K-(cit12)/*[position()=1]) 2010; 83 Frieler (C5AY01761K-(cit19)/*[position()=1]) 2009; 877 Hajek (C5AY01761K-(cit6)/*[position()=1]) 2006; 108 Jung (C5AY01761K-(cit17)/*[position()=1]) 2007; 46 Silva (C5AY01761K-(cit21)/*[position()=1]) 2011; 401 Maruta (C5AY01761K-(cit15)/*[position()=1]) 2012; 91 Dong (C5AY01761K-(cit18)/*[position()=1]) 2014; 957 Cole (C5AY01761K-(cit4)/*[position()=1]) 2008 Keera (C5AY01761K-(cit20)/*[position()=1]) 2011; 90 Li (C5AY01761K-(cit8)/*[position()=1]) 2011; 30 Ribeiro (C5AY01761K-(cit2)/*[position()=1]) 2013; 106 Li (C5AY01761K-(cit5)/*[position()=1]) 2012; 31 Bansal (C5AY01761K-(cit3)/*[position()=1]) 2008; 87 Lourenco (C5AY01761K-(cit14)/*[position()=1]) 2009; 79 Bondioli (C5AY01761K-(cit11)/*[position()=1]) 2005; 107 Blacksmith (C5AY01761K-(cit7)/*[position()=1]) 2013; 7 Goncalves Filho (C5AY01761K-(cit10)/*[position()=1]) 2007; 1154 Wipf (C5AY01761K-(cit16)/*[position()=1]) 1993; 58 |
References_xml | – volume: 18 start-page: 9 year: 2011 ident: C5AY01761K-(cit1)/*[position()=1] publication-title: Ecol. Chem. Eng. S contributor: fullname: Stelmachowski – volume: 30 start-page: 1167 year: 2011 ident: C5AY01761K-(cit8)/*[position()=1] publication-title: Fenxi Ceshi Xuebao contributor: fullname: Li – volume: 106 start-page: 23 year: 2013 ident: C5AY01761K-(cit2)/*[position()=1] publication-title: Microchem. J. doi: 10.1016/j.microc.2012.04.011 contributor: fullname: Ribeiro – volume: 89 start-page: 21 year: 2012 ident: C5AY01761K-(cit13)/*[position()=1] publication-title: Talanta doi: 10.1016/j.talanta.2011.10.055 contributor: fullname: Lima – volume: 83 start-page: 559 year: 2010 ident: C5AY01761K-(cit12)/*[position()=1] publication-title: Talanta doi: 10.1016/j.talanta.2010.09.061 contributor: fullname: Silva – volume: 107 start-page: 153 year: 2005 ident: C5AY01761K-(cit11)/*[position()=1] publication-title: Eur. J. Lipid Sci. Technol. doi: 10.1002/ejlt.200401054 contributor: fullname: Bondioli – volume: 1154 start-page: 477 year: 2007 ident: C5AY01761K-(cit10)/*[position()=1] publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2007.04.063 contributor: fullname: Goncalves Filho – volume: 31 start-page: 36 year: 2012 ident: C5AY01761K-(cit5)/*[position()=1] publication-title: Fenxi Shiyanshi contributor: fullname: Li – volume: 957 start-page: 30 year: 2014 ident: C5AY01761K-(cit18)/*[position()=1] publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2014.02.054 contributor: fullname: Dong – volume: 108 start-page: 666 year: 2006 ident: C5AY01761K-(cit6)/*[position()=1] publication-title: Eur. J. Lipid Sci. Technol. doi: 10.1002/ejlt.200600004 contributor: fullname: Hajek – volume: 87 start-page: 3369 year: 2008 ident: C5AY01761K-(cit3)/*[position()=1] publication-title: Fuel doi: 10.1016/j.fuel.2008.04.033 contributor: fullname: Bansal – start-page: 44 year: 2008 ident: C5AY01761K-(cit4)/*[position()=1] publication-title: LC GC Eur. contributor: fullname: Cole – volume: 7 start-page: 1419 year: 2013 ident: C5AY01761K-(cit7)/*[position()=1] publication-title: Am. Chem. Soc. contributor: fullname: Blacksmith – volume: 58 start-page: 5880 year: 1993 ident: C5AY01761K-(cit16)/*[position()=1] publication-title: J. Org. Chem. doi: 10.1021/jo00074a006 contributor: fullname: Wipf – volume: 46 start-page: 2616 year: 2007 ident: C5AY01761K-(cit17)/*[position()=1] publication-title: Angew. Chem. doi: 10.1002/anie.200604977 contributor: fullname: Jung – volume: 877 start-page: 3667 year: 2009 ident: C5AY01761K-(cit19)/*[position()=1] publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2009.09.015 contributor: fullname: Frieler – volume: 401 start-page: 365 year: 2011 ident: C5AY01761K-(cit21)/*[position()=1] publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-011-5058-4 contributor: fullname: Silva – volume: 697 start-page: 461 year: 1995 ident: C5AY01761K-(cit9)/*[position()=1] publication-title: J. Chromatogr. A doi: 10.1016/0021-9673(94)00867-9 contributor: fullname: Plank – volume: 79 start-page: 92 year: 2009 ident: C5AY01761K-(cit14)/*[position()=1] publication-title: Talanta doi: 10.1016/j.talanta.2009.03.013 contributor: fullname: Lourenco – volume: 91 start-page: 187 year: 2012 ident: C5AY01761K-(cit15)/*[position()=1] publication-title: Fuel doi: 10.1016/j.fuel.2011.06.071 contributor: fullname: Maruta – volume: 90 start-page: 42 year: 2011 ident: C5AY01761K-(cit20)/*[position()=1] publication-title: Fuel doi: 10.1016/j.fuel.2010.07.046 contributor: fullname: Keera |
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SubjectTerms | Arrays Biodiesel Byproducts Chlorides Copper chlorides Diodes Glycerols Mathematical analysis Transesterification |
Title | Sensitive determination of glycerol by derivatization using a HPLC-DAD method in biodiesel samples |
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