Sensitive determination of glycerol by derivatization using a HPLC-DAD method in biodiesel samples
Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through...
Saved in:
Published in | Analytical methods Vol. 7; no. 18; pp. 7805 - 7810 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Biodiesel is mainly produced through a transesterification reaction which yields biodiesel and glycerol, a by-product. This article describes the quantification of glycerol based on high performance liquid chromatography (HPLC) using a diode array detector (DAD) in various biodiesel samples through post-derivatization. Glycerol was converted into a UV active product
i.e.
glyceryl tribenzoate (GTB) through a simple and effective esterification reaction using benzoyl chloride and copper chloride as the catalyst under mild conditions. Optimized reaction conditions (affording a 90% yield) were obtained by using copper chloride (17 mol%), benzoyl chloride (12.0 equiv.) and triethyl amine (11.0 equiv.). The limit of detection and limit of quantification were found to be 0.23 μg mL
−1
and 0.76 μg mL
−1
, respectively. The developed and validated HPLC-DAD method is sensitive, selective, reproducible and successfully applied for the quantification of glycerol in biodiesel from various sources. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/C5AY01761K |