Undernutrition alters the hypothalamic ganglioside profile of the fetal rat

• Published in: The Journal of nutritional biochemistry Vol. 6; no. 3; pp. 155 - 157
• Main Authors: Trindade, Vera M.T., Scotti, Luciana, Becker, Daniela, Perry, Marcos L.S., Bernard, E.A.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.1995; Elsevier Science
• Subjects: Biological and medical sciences; CARENCE PROTEIQUE; CEREBRO; DEFICIENCIA DE PROTEINAS; DIETA; ENCEPHALE; EXPERIMENTACION; EXPERIMENTATION; FETO; FOETUS; ganglioside; GLICOLIPIDOS; GLYCOLIPIDE; hypothalamus; INGESTION DE ALIMENTOS; MALNUTRICION; MALNUTRITION; Medical sciences; Metabolic diseases; MODELE ANIMAL; MODELOS ANIMALES; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...); PRISE ALIMENTAIRE (HOMME); protein undernutrition; PROTEINAS; PROTEINE; RAT; RATA; REGIME ALIMENTAIRE; protein undernutrition; ganglioside; hypothalamus; Embryonic development; Rat; Rodentia; Central nervous system; Biosynthesis; Hypothalamus; Metabolism; Feeding; Proteins; Vertebrata; Mammalia; Ganglioside; Animal; Sphingoglycolipid; Fetus; Malnutrition; Restricted diet; Nutritional status; Nutritional deficiency; Brain (vertebrata)
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/0955-2863(94)00012-B

In order to investigate the effect of protein undernutrition on the gangliosides profile, hypothalami of control and undernourished fetal rats were incubated with [6− 3H]glucosamine. Tissues were extracted with chloroform: methanol, and the purified ganglioside fractions were chromatographed on TLC plates. Chromatograms from both control and undernourished rats showed the presence of ganglioside bands that comigrated as G M1, G D3, G D1a, G D1b, G T1b, and G Q1b. When the two chromatograms were compared in terms of radioactive ganglioside distribution, the G D3 label was found to be higher in the hypothalamus of undernourished rats while the G D1a label was higher in the hypothalamus of control rats. Since G D3 predominates during neuroblast and glioblast proliferation, while G D1a is important during synaptogenesis, the present data may reflect a delay in hypothalamic development determined by protein undernutrition.