Revealing surface changes associated with maturation of ram spermatozoa by centrifugal counter-current distribution in an aqueous two-phase system

• Published in: Journal of Chromatography A Vol. 668; no. 1; pp. 173 - 178
• Main Authors: Ollero, M., Pascual, M.L., Muiñio-Blanco, T., Cebrián-Pérez, J.A., López-Pérez, M.J.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 06.05.1994; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Membrane - chemistry; Cellular Senescence; Centrifugation; Countercurrent Distribution; Dextrans; Diverse techniques; Electrochemistry; Fundamental and applied biological sciences. Psychology; Male; Molecular and cellular biology; Polyethylene Glycols; Sheep; Spermatozoa - chemistry; Spermatozoa - ultrastructure; Water; Heterogeneity; Separation method; Vertebrata; Spermatozoa; Mammalia; Centrifuging; Countercurrent; Sheep; Artiodactyla; Cell surface; Ungulata
• ISSN: 0021-9673
• DOI: 10.1016/0021-9673(94)80106-1

Centrifugal counter-current distribution (CCCD) in an aqueous two-phase system was used to detect changes associated with maturation of ejaculated ram spermatozao. Spermatozoa obtained form three successive ejaculates of rams maintained in abstinence for one, two and three days were fractionated by CCCD. The results show that these ejaculates are relatively enriched in a cell population which presents a very high enhanced affinity to the lower dextran-rich phase. This cell population is not associated with loss of acrosomal integrity. In addition, it tends to disappear with longer abstinence periods, or after successive ejaculations at the same abstinence period, strongly suggesting that it is composed of immature cells. Therefore, phase partitioning can detect surface changes accompanying sperm maturation and offers a new possibility for sperm quality analysis.