An improved preservation technique for cells of hemopoietic origin

A new technique for the cryopreservation of cell lines of hemopoietic origin is described. It uses a combination of 10% polyvinylpyrrolidone or hydroxyethylstarch with 15% glycerol, a slow stepwise addition of the cryoprotectants before freezing, cooling at 4 °C/min, rapid rewarming, and a slow step...

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Bibliographic Details
Published inCryobiology Vol. 22; no. 5; pp. 495 - 498
Main Authors Conscience, Jean-françois, Fischer, Franz
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.10.1985
Elsevier
Subjects
Animals
Biological and medical sciences
Cell Line
Cell Survival
Diverse techniques
Freezing
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - cytology
Mice
Molecular and cellular biology
Rats
Time Factors
Tissue Preservation - methods
Vertebrata
Cell line
Mammalia
Cryopreservation
Hematopoietic cell
Cryoprotective agent
Method
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Summary:A new technique for the cryopreservation of cell lines of hemopoietic origin is described. It uses a combination of 10% polyvinylpyrrolidone or hydroxyethylstarch with 15% glycerol, a slow stepwise addition of the cryoprotectants before freezing, cooling at 4 °C/min, rapid rewarming, and a slow step-wise dilution after thawing. This technique has given improved recovery rates with a number of basophil/mast cell lines, as well as a wide range of hemopoietic cells including hybridomas.
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content type line 23
ISSN:0011-2240
1090-2392
DOI:10.1016/0011-2240(85)90162-2