Activation of biosynthesis of guanyl-specific ribonuclease secreted by Bacillus circulans under salt stress

• Published in: Molecular biology (New York) Vol. 50; no. 6; pp. 874 - 879
• Main Authors: Kharitonova, M. A., Kipenskaya, L. V., Ilinskaya, O. N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.11.2016; Springer Nature B.V
• Subjects: Abiotic stress; Bacillus; Bacillus circulans; Biochemistry; Biomedical and Life Sciences; Biosynthesis; Human Genetics; Life Sciences; Molecular biology; Molecular Cell Biology; Salt; biosynthesis activation; salt stress; guanyl-specific ribonuclease
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893316050083

The gene transcription of guanyl-specific ribonucleases (RNases), which provide available phosphate to cells of Bacillus , is controlled by the signal transduction system PhoP‒PhoR. However, the biosynthesis of B. circulans RNase does not depend on the signal-transduction regulatory proteins of Pho regulon. It has been found that raising the salt molar concentration in culture medium increases the level of extracellular guanyl-specific ribonuclease Bci synthesized by B. circulans . Sequences homologous to the binding sites of the regulatory protein DegU were found in RNase Bci promoter. The functioning of the DegS–DegU signal transduction system is stimulated by a high salt concentration. Using a strain of B. subtilis that is defective in the DegU regulatory protein, we have shown that the DegS–DegU system participates in the regulation of RNase Bci expression under salt stress.