Isolation of Lactococcus garvieae NEP21 from raw cow (Bos indicus) milk in Nepal

Lactococcus garvieae is an emerging zoonotic pathogen impacting both humans and animals. Infection of this bacterium is known to cause mastitis in cattle, and endocarditis, osteomyelitis, liver abscess, and gastrointestinal problems are reported in immunocompromised and elderly people that regularly...

Full description

Saved in:
Bibliographic Details
Published inThe Science of the total environment Vol. 861; p. 160641
Main Authors Dawadi, Prabin, Odari, Ranjeeta, Poudel, Ram Chandra, Pokhrel, Lok R., Bhatt, Lok Ranjan
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 25.02.2023
Subjects
Aged
Animals
Cattle
Emerging zoonoses
Female
Humans
ITS sequencing
Lactic acid bacteria
Lactococcus - genetics
Lactococcus garvieae
Meat
Milk
Nepal
Nepal
Lactic acid bacteria
ITS sequencing
Lactococcus garvieae
Emerging zoonoses
Milk
Online AccessGet full text

Cover

More Information
Summary:Lactococcus garvieae is an emerging zoonotic pathogen impacting both humans and animals. Infection of this bacterium is known to cause mastitis in cattle, and endocarditis, osteomyelitis, liver abscess, and gastrointestinal problems are reported in immunocompromised and elderly people that regularly consume or handle raw meat, milk, dairy products, and seafood. This study aimed at investigating and detecting lactic acid bacteria in raw cow (Bos indicus) milk samples from a smallholder farm in Nepal. Based on the plate culture, biochemical tests, and molecular sequencing of 16 s ribosomal RNA coding nuclear DNA region followed by phenotypic and genotypic analyses, L. garvieae NEP21 was detected and identified for the first time in Nepal in raw cow milk samples. This finding suggests the prevalence of L. garvieae NEP21 in raw cow milk and recommends further research and surveillance for understanding the extent of its presence in Nepal and globally for informed management of its infection in cattle and humans. [Display omitted] •Lactococcus garvieae is an emerging zoonotic pathogen impacting humans and animals•First report of Lactococcus garvieae NEP21 isolated from raw cow milk in Nepal•Phenotypic and genotypic methods enabled the isolation, detection, and naming of L. garvieae NEP21•Further research is recommended for understanding the extent of L. garvieae NEP21 prevalence in Nepal and globally
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2022.160641