Study on the Effect of Type III Recombinant Humanized Collagen on Human Vascular Endothelial Cells

The effect and mechanism of type III recombinant humanized collagen (hCOLIII) on human vascular endothelial EA.hy926 cells at the cellular and molecular levels were investigated. The impact of hCOLIII on the proliferation of EA.hy926 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet...

Full description

Saved in:
Bibliographic Details
Published inTissue engineering. Part C, Methods Vol. 30; no. 2; p. 53
Main Authors Wang, Han, Xiao, Yonghao, Zhang, Yuanguo, Meng, Zhu, Zhao, Chenyu, Qiu, Fanshan, Li, Chongchong, Feng, Zengguo
Format Journal Article
LanguageEnglish
Published United States 01.02.2024
Subjects
migration
vascular endothelial cells
proliferation
recombinant human type III collagen
VEGF
angiogenesis
Online AccessGet more information

Cover

More Information
Summary:The effect and mechanism of type III recombinant humanized collagen (hCOLIII) on human vascular endothelial EA.hy926 cells at the cellular and molecular levels were investigated. The impact of hCOLIII on the proliferation of EA.hy926 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid assay, the effect of hCOLIII on cell migration was investigated by scratch assay, the impact of hCOLIII on cell cycle and apoptosis was detected by flow cytometry, the ability of hCOLIII to induce angiogenesis of EA.hy926 cells was evaluated by angiogenesis assay, and the effect of hCOLIII on vascular endothelial growth factor (VEGF) expression was detected by real-time reverse transcription-polymerase chain reaction analysis. The hCOLIII at concentrations of 0.5, 0.25, and 0.125 mg/mL all showed specific effects on the proliferation and migration of human vascular endothelial cells. It could also affect the cell cycle, increase the proliferation index, and increase the expression level of VEGF in human vascular endothelial cells. In the meantime, hCOLIII at the concentration of 0.5 mg/mL also showed a promoting effect on vessel formation. hCOLIII can potentially promote the endothelization process of blood vessels, mainly by affecting the proliferation, migration, and vascular-like structure of human endothelial cells. At the same time, hCOLIII can promote the expression of VEGF. This collagen demonstrated its potential as a raw material for cardiovascular implants.
ISSN:1937-3392
DOI:10.1089/ten.TEC.2023.0211