Evaluation of PDTC for removing Ni(II) from human serum transferrin in vitro

Pyrrolidine dithiocarbamate (PDTC)can be an oral chelator with pKa=3.300+/-0.002. It behaves as a bidentate ligand at serum pH. The effect of pH on Ni2+-Tf indicated that the maximum adsorption was at pH=7.4. The effective Ni-PDTC binding constant was determined (logk=11.1+/-0.1) for the 1:2 Ni(PDTC...

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Bibliographic Details
Published inInternational journal of artificial organs Vol. 29; no. 8; p. 809
Main Authors Hooshmand, A, Badiei, A, Fatemi, S J
Format Journal Article
LanguageEnglish
Published United States 01.08.2006
Subjects
Adsorption
Chelating Agents - pharmacology
Humans
Hydrogen-Ion Concentration
Nickel - antagonists & inhibitors
Nickel - blood
Protein Binding - drug effects
Pyrrolidines - pharmacology
Spectrophotometry, Ultraviolet
Temperature
Thiocarbamates - pharmacology
Titrimetry
Transferrin - metabolism
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Summary:Pyrrolidine dithiocarbamate (PDTC)can be an oral chelator with pKa=3.300+/-0.002. It behaves as a bidentate ligand at serum pH. The effect of pH on Ni2+-Tf indicated that the maximum adsorption was at pH=7.4. The effective Ni-PDTC binding constant was determined (logk=11.1+/-0.1) for the 1:2 Ni(PDTC)2 complex using UV-vis spectra. The isosbestic point at 298 indicated that the complexation reaction was done directly (without side reaction). Removal of Ni from transferrin (Tf) was investigated by reverse titration of PDTC at 25 masculineC and pH=7.4 using UV-vis spectra. PDTC is able to remove 25% of Ni from human serum transferrin.
ISSN:0391-3988
DOI:10.1177/039139880602900811