The effect of exercise and in vivo treatment with ACTH and norepinephrine on the lipolytic responsiveness of guinea pig ( Cavia porcellus) adipose tissue

• Published in: Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 101; no. 4; pp. 553 - 557
• Main Authors: Van den Bergh, Ryno, Oelofsen, Willem, Naudé, Ryno J., Terblanche, Stephanus E.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.04.1992; Elsevier Science
• Subjects: Adipose Tissue - drug effects; Adipose Tissue - metabolism; Adipose Tissue - physiology; Adrenocorticotropic Hormone - administration & dosage; Adrenocorticotropic Hormone - physiology; Animals; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Guinea Pigs; In Vitro Techniques; Kinetics; Lipolysis - drug effects; Lipolysis - physiology; Male; Norepinephrine - administration & dosage; Norepinephrine - physiology; Physical Conditioning, Animal; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue; cAMP, Cyclic adenosine 3′,5′-monophosphate; KRBG, Krebs-Ringer bicarbonate glucose buffer; NE, norepinephrine; BSA, bovine serum albumin; MSH, melanocyte-stimulating hormone; PCA, perchloric acid; TSH, thyroid-stimulating hormone; FFA, free fatty acids; ACTH, adrenocorticotrophic hormone; DHE, dihydroergotamine; Physical exercise; Vertebrata; Mammalia; Guinea pig; Adipose tissue; Adenohypophyseal hormone; Rodentia; ACTH; Norepinephrine; Catecholamine; Lipolysis
• ISSN: 0305-0491
• DOI: 10.1016/0305-0491(92)90337-Q

1. 1. In adipose slices of the guinea pig ( Cavia porcellus), adrenocorticotrophic hormone (ACTH) produced a significantly higher lipolytic response (increased V max and decreased K m ) as compared to norepinephrine (NE), thus indicating a marked difference with human adipose tissue. 2. 2. Exercise-training caused a significant increase in the affinity of the lipolytic response in adipose tissue towards both ACTH and NE, but had no significant effect on V max . 3. 3. Prolonged in vivo treatment with both ACTH and NE significantly decreased the responsiveness (affinity and capacity) of adipose slices towards ACTH. Responsiveness towards NE was much less affected.