Noninvasive diagnostics of bladder cancer based on analysis of telomerase activity and expression hTERT and hTR coding its subunits

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 8; no. 1; pp. 59 - 68
• Main Authors: Glukhov, A. I., Grigorieva, Y. E., Gordeev, S. A., Vinarov, A. Z., Potoldykova, N. V.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 2014; Springer Nature B.V
• Subjects: Biochemistry; Bioorganic Chemistry; Bladder cancer; Chemistry; Chemistry and Materials Science; Gene expression; Medicinal Chemistry; telomerase RNA-subunit (hTR); bladder cancer (BC); diagnositics; noninvasive diagnostics; telomerase; telomerase catalytic subunit (hTERT)
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750814010053

In order to develop noninvasive diagnostics of bladder cancer (BC), telomerase activity has been examined by means the TRAP method (telomerase repeat amplification protocol) in tumor tissue and urine pellet samples taken from patients with bladder cancer. The levels of relative expression of genes encoding telomerase catalytic subunit (hTERT) and its RNA subunit (hTR) were evaluated by RT-PCR. Telomerase activity and expression of genes encoding its subunits were detected in both tumor tissues and in the urine cell pellet from each BC patient. Results of our study demonstrate possibility of noninvasive BC diagnostics using combination of these methods with sensitivity of 96% and specificity of 100% in the case of telomerase detection and with sensitivity of 80% and specificity of 100% in the case of hTERT detection in urine pellet samples.