Three generation toxicity study of butylated hydroxytoluene administered to mice

• Published in: Toxicology letters Vol. 66; no. 3; pp. 295 - 304
• Main Authors: Toyohito, Tanaka, Shinshi, Oishi, Osamu, Takahashi
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.03.1993; Amsterdam Elsevier Science
• Subjects: Animals; Antioxidants - toxicity; Behavior, Animal - drug effects; Behavioural toxicity; Biological and medical sciences; Body Weight - drug effects; Butylated hydroxytoluene; Butylated Hydroxytoluene - analogs & derivatives; Butylated Hydroxytoluene - toxicity; Central Nervous System - drug effects; Developmental toxicity; Female; Food toxicology; Lactation; Litter Size - drug effects; Male; Medical sciences; Mice; Reproduction - drug effects; Reproductive toxicity; Toxicology; Behavioural toxicity; Mice; Developmental toxicity; Reproductive toxicity; Butylated hydroxytoluene; Reproduction diseases; Toxicity; Rodentia; Multigeneration study; Food additive; Antioxidant; BHT; Vertebrata; Mammalia; Mouse; Animal; Development; Behavior; Teratogen
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/0378-4274(93)90011-L

Butylated hydroxytoluene (BHT) was administered to mice, from 5 weeks of age of the F 0 generation to the weaning of the F 2 generation, in the diet at levels of 0 (control), 0.015, 0.045, 0.135, and 0.405%, and some reproductive and neurobehavioural parameters were measured. There was no consistent significant effect on number of litters, litter size, and litter weight at birth for each generation. The body weight of the pups of the 0.015% BHT group was increased at birth and during the lactation period for each generation. In the F 2 generation, the body weights of the pups of other treatment groups were increased during the lactation period, particularly at postnatal day (PND) 21. In the neurobehavioural parameters, a few parameters were increased in treatment groups; i.e., surface righting at PND 7 in the F 1 generation, surface righting at PND 4 and negative geotaxis at PND 7 in the F 2 generation. The dose levels of BHT in this study showed little adverse effect on reproductive and neurobehavioural parameters on mice.