Inhibitory effects of Chinese quince fruit proanthocyanidins with different polymerisation degrees on the formation of heterocyclic aromatic amines in chemical model systems

• Published in: International journal of food science & technology Vol. 57; no. 1; pp. 330 - 341
• Main Authors: Wang, Shou‐Tao, Zhuo, Wen‐Ling, Dan, Ya‐Qian, Qin, Zhao, Zhang, Chen‐Xia, Xi, Jun, Liu, Hua‐Min, Ma, Yu‐Xiang, Wang, Xue‐De
• Format: Journal Article
• Language: English
• Published: Oxford Wiley Subscription Services, Inc 01.01.2022
• Subjects: Additives; Amines; Chinese quince; Degree of polymerization; different polymerisation degree; Ethanol; Fruits; Heterocyclic amines; heterocyclic aromatic amines; High-performance liquid chromatography; inhibition; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Polymerization; Proanthocyanidins
• ISSN: 0950-5423; 1365-2621
• DOI: 10.1111/ijfs.15409

Summary Proanthocyanidins with different polymerisation degrees were extracted from Chinese quince fruits by subcritical ethanol/water into four fractions (F1‐F4), which were then analysed using UPLC‐MS/MS, GPC and thiolysis combined with RP‐HPLC MS and MALDI‐TOF MS (matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry). The inhibitory effects of these proanthocyanidins on the formation of heterocyclic aromatic amines (HCAs), such as norharman, harman and PhIP, in chemical model systems were determined. The results reflected that the mean degree of polymerisation (DP) of proanthocyanidins in fractions F1, F2, F3 and F4 were 8.0, 12.7, 27.5 and 64.9, respectively. All four fractions prominently and dose‐dependently reduced the formation of HCAs at concentrations of 0.5, 1 and 2 mg mL−1 in chemical models. F1, the fraction richest in oligomers, had the most effective inhibition of harman, norharman and PhIP, with the levels reduced by 65.71%, 37.15% and 72.99%, respectively, when used at a concentration of 2 mg mL−1. The mean DP of proanthocyanidins showed negative correlation with the inhibition of HCA formation (R2= −0.697 for norharman, −0.799 for PhIP and −0.758 for harman). These findings suggest that proanthocyanidins from Chinese quince fruit have the potential to be used as additives to reduce the levels of heterocyclic amines in foods. Proanthocyanidins with different polymerization degrees, which were extracted from Chinese quince fruits, can effectively prevent the formation of heterocyclic aromatic amines in chemical model systems.