Adrenal gland SNAP-25 expression is altered in thyroid hormone receptor knock-out mice

• Published in: Neuroreport Vol. 12; no. 7; p. 1427
• Main Authors: Hepp, R, Grant, N J, Espliguero, G, Aunis, D, Sarlieve, L L, Rodrigues-Pena, A, Langley, K
• Format: Journal Article
• Language: English
• Published: England 25.05.2001
• Subjects: Adrenal Medulla - cytology; Adrenal Medulla - growth & development; Adrenal Medulla - metabolism; Animals; Antigens, Surface - metabolism; Catecholamines - biosynthesis; Cerebellum - cytology; Cerebellum - growth & development; Cerebellum - metabolism; Chromaffin Cells - cytology; Chromaffin Cells - metabolism; Chromogranin A; Chromogranins - metabolism; Gene Expression Regulation, Developmental - drug effects; Gene Expression Regulation, Developmental - physiology; Membrane Proteins - drug effects; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Mice, Knockout; Nerve Tissue Proteins - drug effects; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; PC12 Cells - cytology; PC12 Cells - drug effects; PC12 Cells - metabolism; Peptide Fragments - metabolism; Protein Transport - genetics; R-SNARE Proteins; Rats; Receptors, Thyroid Hormone - deficiency; Receptors, Thyroid Hormone - genetics; RNA, Messenger - metabolism; Synaptic Membranes - genetics; Synaptic Membranes - metabolism; Synaptosomal-Associated Protein 25; Syntaxin 1; Triiodothyronine - metabolism; Triiodothyronine - pharmacology
• ISSN: 0959-4965
• DOI: 10.1097/00001756-200105250-00026

SNAP-25 is a protein in neurons and neuroendocrine cells, which is involved, together with syntaxin and VAMP, in neurotransmitter release and neurite outgrowth. Since the thyroid hormone receptors TR alpha and TR beta are essential for nervous system development, their possible role in regulating the expression of these vesicle trafficking proteins was examined by analysing SNAP-25 levels in TR alpha and TR beta knock-out mice. Immunoblotting and RT-PCR showed that SNAP-25 levels are increased in the adrenal gland, but not in cerebellum, in knock-out mice, while syntaxin-1 and VAMP-2 are unaffected in either tissue. Treatment of the pheochromocytoma-derived cell line PC12 with the thyroid hormone L-3,5,3'-triiodothyronine (T3) decreased SNAP-25 expression. Together, these data suggest that thyroid hormones exert a negative regulatory effect on SNAP-25 in adrenal medullary neuroendocrine cells.