Metabolic pathway of anaerobic ammonium oxidation on the basis of 15N studies in a fluidized bed reactor

• Published in: Microbiology (Society for General Microbiology) Vol. 143; no. 7; pp. 2415 - 2421
• Main Authors: van de Graaf, Astrid A, de Bruijn, Peter, Robertson, Lesley A, Jetten, Mike S. M, Kuenen, J. Gijs
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.1997; Society for General Microbiology
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Anaerobiosis; Fluidized bed reactor; Microbial activity; Ammonium; Bioreactor; Hydroxylamine; Batchwise; Nitrites; Denitrification; Oxidation; Metabolic pathway; Hydrazine
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-143-7-2415

Kluyver Laboratory for Biotechnology, Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands *Author for correspondence: Mike S. M. Jetten. Tel: +31 15 2781193. Fax: +31 15 2782355. e-mail: M.Jetten@STM.TUDelft.NL ABSTRACT Summary: A novel metabolic pathway for anaerobic ammonium oxidation with nitrite as the electron acceptor has been elucidated using 15 N-Iabelled nitrogen compounds. These experiments showed that ammonium was biologically oxidized with hydroxylamine as the most probable electron acceptor. The hydroxylamine itself is most likely derived from nitrite. Batch experiments in which ammonium was oxidized with hydroxylamine transiently accumulated hydrazine. The conversion of hydrazine to dinitrogen gas is postulated as the reaction generating electron equivalents for the reduction of nitrite to hydroxylamine. During the conversion of ammonium, a small amount of nitrate was formed from some of the nitrite. The addition of NH 2 OH to an operating fluidized bed system caused a stoichiometric increase in the ammonium conversion rate (1 mmol I -1 h -1 ) and a decrease in the nitrate production rate (0.5 mmol I -1 h -1 ). Addition of hydrazine also caused a decrease in nitrate production. On the basis of these findings, it is postulated that the oxidation of nitrite to nitrate could provide the anaerobic ammonium-oxidizing bacteria with the reducing equivalents necessary for CO 2 fixation. Keywords: ammonium oxidation, hydroxylamine, hydrazine, mass spectrometry Present address: IMPULS Science & Technology Center, PO Box 421, 1000 AK Amsterdam, The Netherlands.