Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil

• Published in: Emerging infectious diseases Vol. 23; no. 11; pp. 1867 - 1871
• Main Authors: Fischer, Carlo, Torres, Maria C, Patel, Pranav, Moreira-Soto, Andres, Gould, Ernest A, Charrel, Rémi N, de Lamballerie, Xavier, Nogueira, Rita Maria Ribeiro, Sequeira, Patricia C, Rodrigues, Cintia D S, Kümmerer, Beate M, Drosten, Christian, Landt, Olfert, Bispo de Filippis, Ana Maria, Drexler, Jan Felix
• Format: Journal Article
• Language: English
• Published: United States Centers for Disease Control and Prevention 01.11.2017
• Subjects: Americas; Brazil - epidemiology; Disease Outbreaks; Dispatch; Humans; Lineage-Specific Real-Time Reverse Transcription-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil; Real-Time Polymerase Chain Reaction - methods; real-time RT-PCR; Reverse Transcriptase Polymerase Chain Reaction - methods; Species Specificity; surveillance; vaccine safety; vector-borne infections; Yellow Fever - epidemiology; Yellow Fever - virology; Yellow Fever Vaccine - isolation & purification; yellow fever virus; Yellow fever virus - isolation & purification; vaccine safety; Americas; viruses; vector-borne infections; yellow fever virus; reverse transcription PCR; Brazil; surveillance; real-time RT-PCR
• ISSN: 1080-6040; 1080-6059; 1080-6059
• DOI: 10.3201/eid2311.171131

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.