Increased Brucella abortus asRNA_0067 expression under intraphagocytic stressors is associated with enhanced virB2 transcription
Intracellular pathogens like Brucella face challenges during the intraphagocytic adaptation phase, where the modulation of gene expression plays an essential role in taking advantage of stressors to persist inside the host cell. This study aims to explore the expression of antisense virB2 RNA strand...
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Published in | Archives of microbiology Vol. 206; no. 6; p. 285 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.06.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Intracellular pathogens like
Brucella
face challenges during the intraphagocytic adaptation phase, where the modulation of gene expression plays an essential role in taking advantage of stressors to persist inside the host cell. This study aims to explore the expression of antisense
virB2
RNA strand and related genes under intracellular simulation media. Sense and antisense
virB2
RNA strands increased expression when nutrient deprivation and acidification were higher, being starvation more determinative. Meanwhile,
bspB
, one of the T4SS effector genes, exhibited the highest expression during the exposition to pH 4.5 and nutrient abundance. Based on RNA-seq analysis and RACE data, we constructed a regional map depicting the 5' and 3' ends of
virB2
and the cis-encoded asRNA_0067. Without affecting the CDS or a possible autonomous RBS, we generate the deletion mutant ΔasRNA_0067, significantly reducing
virB2
mRNA expression and survival rate. These results suggest that the antisense asRNA_0067 expression is promoted under exposure to the intraphagocytic adaptation phase stressors, and its deletion is associated with a lower transcription of the
virB2
gene. Our findings illuminate the significance of these RNA strands in modulating the survival strategy of
Brucella
within the host and emphasize the role of nutrient deprivation in gene expression. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0302-8933 1432-072X 1432-072X |
DOI: | 10.1007/s00203-024-03984-8 |