Sensitive glucose biosensor based on cyclodextrin modified carbon nanotubes for detecting glucose in honey

• Published in: Journal of food composition and analysis Vol. 105; p. 104221
• Main Authors: Xia, Jiaojiao, Zou, Bin, Liu, Feng, Wang, Pengyun, Yan, Yan
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.01.2022
• Subjects: Accuracy; Biosensor; Excellent stability; Functionalized; glucose; Honey; Immobilization; Multi-walled carbon nanotubes; Oxidase; β-cyclodextrin; Honey; glucose; Accuracy; Excellent stability; Biosensor; Oxidase; Immobilization; Multi-walled carbon nanotubes; Functionalized; β-cyclodextrin
• ISSN: 0889-1575; 1096-0481
• DOI: 10.1016/j.jfca.2021.104221

[Display omitted] •β-cyclodextrin was used to modify the surface of MWCNTs.•The dispersibility of MWCNTs was improved after modification.•GOx was loaded onto β-CD/MWCNTs to prepare glucose biosensor.•The glucose detection limit of GOx-MWCNTs/GCE was 0.42 μM.•Glucose biosensor was successfully applied in the honey sample. As a healthy and nutritious food, the taste of honey is usually affected by glucose crystals. To investigate the glucose concentration of honey, a new electroanalytical method based on a composite material of functionalized MWCNTs with β-cyclodextrin (β-CD/MWCNTs) was hereby proposed. After glucose oxidase (GOx) loading on β-CD/MWCNTs, a new biosensor GOx/β-CD/MWCNTs/GCE was constructed for the detection of glucose in honey. Scanning electron microscopy (SEM) and infrared spectroscopy (FT-IR) proved the successful immobilization of GOx on the β-CD/MWCNTs carrier. The prepared biosensor showed a linear response to the glucose concentration in the range of 50 μM ∼ 1.15 mM with the detection limit of 0.42 μM and a sensitivity of 32.28 μA mM−1 cm−2 was obtained. The biosensor has excellent stability (95.7 % after two weeks), reproducibility (n = 10, relative standard deviation (RSD) = 2.5 %) and anti-interference. HPLC results validated the accuracy of the newly proposed method in the detection of glucose in real honey samples.