Recent advances in electrochemiluminescence-based simultaneous detection of multiple targets

• Published in: TrAC, Trends in analytical chemistry (Regular ed.) Vol. 123; p. 115767
• Main Authors: Lv, Wanxue, Ye, Haochen, Yuan, Zhiqin, Liu, Xuejiao, Chen, Xu, Yang, Wensheng
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2020
• Subjects: Biomarkers; Electrochemiluminescence; Miscellaneous strategies; Multiple targets; Potential-resolved strategy; Simultaneous detection; Spatial-resolved strategy; Spectrum-resolved strategy; Multiple targets; Spectrum-resolved strategy; Simultaneous detection; Potential-resolved strategy; Biomarkers; Electrochemiluminescence; Miscellaneous strategies; Spatial-resolved strategy
• ISSN: 0165-9936; 1879-3142
• DOI: 10.1016/j.trac.2019.115767

The electrochemiluminescence (ECL)-based simultaneous detection of multiple biomarkers in one sample has received considerable attention in the past decade due to the increasing need for accurate cancer diagnosis and prediction. However, there has not been a comprehensive summary of ECL-based simultaneous detection of multiple biomarkers. In this review, we focus on recent advances in multiplex ECL assays using diverse detection strategies and their applications for various biomarkers. Based on analyte-induced changes in ECL signals, systems for the simultaneous quantitation of biomarkers (e.g., microRNAs, proteins, and cancer cells) have been proposed. These systems are classified into the following four categories: spatial-resolved, potential-resolved, spectrum-resolved, and miscellaneous strategies. Finally, future perspectives for multiplex ECL-based simultaneous detection systems are discussed. [Display omitted] •The recent advances in ECL-based multiplex bioanalysis are reviewed.•Simultaneous detection of multiple targets via different strategies is concluded.•Future perspectives for this field are discussed.