CoA Synthase is in complex with p85αPI3K and affects PI3K signaling pathway

The complex interplay between cellular signaling and metabolism in eukaryotic cells just start to emerge. Coenzyme A (CoA) and its derivatives play a key role in cell metabolism and also participate in regulatory processes. CoA Synthase (CoASy) is a mitochondria-associated enzyme which mediates two...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 385; no. 4; pp. 581 - 585
Main Authors Breus, Oksana, Panasyuk, Ganna, Gout, Ivan T., Filonenko, Valeriy, Nemazanyy, Ivan
Format Journal Article
LanguageEnglish
Published Elsevier Inc 07.08.2009
Subjects
CoASy
Coenzyme A
Metabolism
p85αPI3K
PI3K
CoASy
Coenzyme A
PI3K
Metabolism
p85αPI3K
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Summary:The complex interplay between cellular signaling and metabolism in eukaryotic cells just start to emerge. Coenzyme A (CoA) and its derivatives play a key role in cell metabolism and also participate in regulatory processes. CoA Synthase (CoASy) is a mitochondria-associated enzyme which mediates two final stages of de novo CoA biosynthesis. Here, we report that CoASy is involved in signaling events in the cell and forms a functional complex with p85αPI3K in vivo. Importantly, observed interaction of endogenous CoASy and p85αPI3K is regulated in a growth factor dependent manner. Surprisingly, both catalytic p110α and regulatory p85α subunits of PI3K were detected in mitochondrial fraction where mitochondria-localized p85αPI3K was found in complex with CoASy. Unexpectedly, significant changes of PI3K signaling pathway activity were observed in experiments with siRNA-mediated CoASy knockdown pointing on the role of CoA biosynthetic pathway in signal transduction.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.05.102