iTRAQ-based proteomic analysis of the differential effects of digested soy peptides and digested soy protein isolates on Lacticaseibacillus rhamnosus

• Published in: Food bioscience Vol. 43; p. 101296
• Main Authors: Zhang, Chi, Zhang, Yinxiao, Xia, Shaoqi, Zhu, Shuya, Li, Wenhui, Aboelenin, Salama Mostafa, Soliman, Mohamed Mohamed, Li, He, Liu, Xinqi
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2021
• Subjects: iTRAQ analysis; Lacticaseibacillus rhamnosus; Morphology; Soybean peptides; Soybean protein isolates; Lacticaseibacillus rhamnosus; Soybean protein isolates; iTRAQ analysis; Soybean peptides; Morphology
• ISSN: 2212-4292; 2212-4306
• DOI: 10.1016/j.fbio.2021.101296

Lacticaseibacillus rhamnosus (L. rhamnosus) is a probiotic strain that has exhibited probiotic properties in the prevention and treatment of intestinal flora imbalance. In order to investigate the morphological differences and molecular mechanisms underlying the effects of simulated gastrointestinal digested soybean protein isolate (dpro22dpro: digested soybean protein isolate, dpep: digested soybean peptide, M: Man Rogosa Sharpe broth, DAPs: Differentially abundant proteins. dpep/M: dpep sample relative to the M sample, dpro/M: dpro sample relative to the M sample, dpep/dpro: dpep sample relative to the dpro sample.) and digested soybean peptide (dpep) treatment on L. rhamnosus, microscopic observation and iTRAQ-based proteomics and bioinformatics analysis were performed. 1462 proteins were identified, and differentially abundant proteins (DAPs) were screened accurately, including 66 DAPs in the dpep group compared with the Man Rogosa Sharpe broth (M) group and 66 DAPs in the dpep/dpro group. Bioinformatic analysis revealed that DAPs in dpep/M and dpep/dpro mainly differed in cellular carbohydrate and nitrogen compound catabolic processes and transport. Some of the DAPs overlapped in the three comparison groups might be pivotal proteins involved in growth and morphologic changes of the dpep and dpro treatments. •Soy peptides and SPIs after simulated digestion showed effective growth promotion and morphologic change of L. rhamnosus.•66 differentially abundant proteins (DAPs) were found respectively in the comparison of dpep/dpro and dpep/M.•Dpep regulating peptides transportation and metabolism could be potential mechanisms to enhance growth of L. rhamnosus.