Synthesis, characterization and in vitro toxicity assessment of DMPS-capped CdTe quantum dots
Water-soluble CdTe quantum dots capped with 2,3-bis(sulfanyl)propane-1-sulfonate have been prepared and their potential use as a heavy metal fluorescent probe was explored. The work also includes a preliminary analysis of the new QDs cytotoxicity on HeLa and LLC-PK1 cells. The title water-soluble th...
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Published in | Polyhedron Vol. 70; pp. 77 - 84 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
09.03.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Water-soluble CdTe quantum dots capped with 2,3-bis(sulfanyl)propane-1-sulfonate have been prepared and their potential use as a heavy metal fluorescent probe was explored. The work also includes a preliminary analysis of the new QDs cytotoxicity on HeLa and LLC-PK1 cells.
The title water-soluble thiol-capped QDs (quantum dots) were synthesized by reacting CdCl2, DMPS and NaHTe in a water medium [DMPS is sodium 2,3-bis(sulfanyl)propane-1-sulfonate]. The nanoparticles were characterized by UV–Vis, fluorescence and FT-IR spectroscopy, transmission electron microscopy (TEM) and powder XRD measurements. The QDs are spherical, with an average diameter of 3.4±0.7nm and good stability against photooxidation and photobleaching. The influence of a wide number of anions and cations on the fluorescence emission of the nanoparticles has been explored. At a low metal cation concentration (5μM), only Hg(II) produces the total quenching of the QD photoemission. At higher concentrations, Cu(II), Ag(I), Pb(II) and Ni(II) also induce a significant quenching. A preliminary analysis of the QD cytotoxicity on HeLa 229 and LLC-PK1 line cells was performed using the MTT assay. In 2h incubation experiments, high cell-viability was observed, but this parameter decreases significantly after 24h incubation. During this longer incubation period only the LLC-PK1 cells maintained a good metabolic activity when [QDs] is ⩽5μg/mL. Accordingly, fluorescence confocal microscopy indicated that the uptake of the nanoparticles is apparently higher in HeLa cells than in LLC-PK1 cells. |
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ISSN: | 0277-5387 |
DOI: | 10.1016/j.poly.2013.12.034 |