New trans-[Ru(NO)(NO2)(dppb)(o-bdqi)]+ complex as NO donor encapsulated Pluronic F-127 micelles

• Published in: Polyhedron Vol. 218; p. 115770
• Main Authors: Luiz Moreira do Amaral, Matheus, Nascimento, Rebecca D., Silva, Larissa Franco, de Souza Arantes, Evelyn Christine, Graminha, Angelica E., Santana da Silva, Roberto, Ueno, Leonardo T., Bogado, André Luiz, DeFreitas-Silva, Gilson, de Lima, Renata Galvão
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 15.05.2022
• Subjects: Cytotoxic evaluation; Diamine ligand; Diphosphine; Nitric oxide; Nitro/nitrosyl ruthenium complex; Pluronic F-127; Diamine ligand; Diphosphine; Cytotoxic evaluation; Nitro/nitrosyl ruthenium complex; Pluronic F-127; Nitric oxide
• ISSN: 0277-5387
• DOI: 10.1016/j.poly.2022.115770

New phosphine ruthenium complex as nitric oxide donor spontaneously when encapsulated in Pluronic F127 polymer micelle in aqueous medium. [Display omitted] •Synthesis of a new compound in the series diphosphine diimine ruthenium complex trans-[Ru(NO2)(NO)(dppb)(o-bdqi)]Cl2.•Encapsulation of trans-[Ru(NO2)(NO)(dppb)(o-bdqi)]Cl2 complex in Pluronic F-127 micelles (F-127/Ru) using thin-film hydration.•Nitric oxide release from F-127/Ru in aqueous medium.•Cytotoxic effect for trans-[Ru(NO2)(NO)(dppb)(o-bdqi)]Cl2 and encapsulated complex F-127/Ru. Ruthenium complexes that act as nitric oxide (NO) donors show potential cytotoxicity in tumor models. In this work, we proposed the synthesis of the new nitrosyl ruthenium complex trans-[Ru(NO2)(NO)(dppb)(o-bdqi)]Cl2, containing a non-innocent ligand of the o-phenylenediamine type (o-bdqi). The 31P{1H} NMR spectra confirmed that the trans-[Ru(NO2)(NO)(dppb)(o-bqdi)] is the isomer formed. The complex was characterized by elemental analysis, mass spectrometry, UV–vis and infrared spectroscopy and cyclic voltammetry. Natural transition orbitals (NTOs) for trans-[RuCl2(dppb)(o-bdqi)] and trans-[Ru(NO2)(NO)(dppb)(o-bdqi)]Cl2 complex were obtained with TD-DFT/B3LYP methodology to contribute to the assignment of electronic transition. A micellar system (F-127/Ru) produced with Pluronic F-127 copolymer was used as a drug delivery system, due to the hydrophobicity of the complex. The F-127/Ru system showed NO release in aqueous media. The free complex showed higher cytotoxicity than the F-127/Ru system in the studied tumor cells. However, the F-127/Ru system showed a higher selectivity index for lung cells. This shows that the Pluronic F-127 carrier can be a good alternative in the use of hydrophobic nitrosyl ruthenium compounds in the treatment of some types of cancer.