A novel electrochemical aptamer biosensor for lead (Ⅱ) ion detection utilizing GO/PAMAM @Au and Au/MB as dual signal amplifiers

• Published in: Journal of food composition and analysis Vol. 131; p. 106274
• Main Authors: Du, Junhui, Liu, Haibin, Chen, Hongshuo, Cui, Chuanjin, Zhang, Wensi, Zhang, Xuechao, Bin, Zhang
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.07.2024
• Subjects: Aptamer biosensors; Bioprobe; Electrochemical; Lead ion; MB-GNPs-ssDNA; PAMAM@Au; Lead ion; MB-GNPs-ssDNA; PAMAM@Au; Bioprobe; Aptamer biosensors; Electrochemical
• ISSN: 0889-1575; 1096-0481
• DOI: 10.1016/j.jfca.2024.106274

A competitive electrochemical aptamer biosensor for the selective detection of Pb2+ was developed in this study. The PAMAM@Au hybrid materials and the bioprobe MB-GNPs-ssDNA were synthesized with chemical methods. Modified electrode with carboxylated graphene oxide was prepared via electrodeposition. PAMAM@Au was immobilized on the modified electrode using the EDC/NHS crosslinker, and aptamer (Apt) was bound to PAMAM@Au hybrid materials using the glutaraldehyde crosslinker. The assembly of MB-GNPs-ssDNA/Apt/PAMAM@Au/GO/SPCE biosensors was achieved through immobilizing bioprobe onto electrodes, based on the theory of aptamer-complementary chain reactions. The fabricated biosensor was characterized through electrochemical experiments, SEM, AFM, TEM, XPS and FTIR. The biosensor's performance was evaluated in a series of Pb2+ aqueous solutions by differential pulse voltammetry (DPV), and the results demonstrated this biosensor had excellent repeatability, stability, and specificity. The detection range spanned from 0.5 pM/L to 500 nM/L, with a detection limit of 5 fM/L. Consequently, the proposed biosensor can serve as a reliable analytical tool for the sensitive detection of lead ions. •A novel competitive electrochemical aptamer biosensor for detecting lead ions was Designed.•The polyamidoamine (PAMAM) dendrimer encapsulated with nanogold was innovatively synthesized.•A biosignal probe MB-GNPs-ssDNA for enhancing the detection signal of biosensor was synthesized.•The redox property of methylene blue was employed to generate a detectable electrical signal.