Fish intended for human consumption: from DNA barcoding to a next-generation sequencing (NGS)-based approach

• Published in: Current opinion in food science Vol. 42; pp. 86 - 92
• Main Authors: Franco, Carlos Manuel, Ambrosio, Rosa Luisa, Cepeda, Alberto, Anastasio, Aniello
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2021
• ISSN: 2214-7993; 2214-8000
• DOI: 10.1016/j.cofs.2021.05.005

•Fish species identification using DNA barcoding is not suitable for mixed fish products.•NGS systems can detect more than 15 mixed fish species in the same food.•NGS systems could control several different characteristics in mixed fish products at same time. In food authentication research, species identification was first developed by comparing the sequences of several mitochondrial genes isolated from food with NCBI or Barcode of Life Database (BOLD) data. These methods usually use Sanger methodology for sequencing although they have difficulty in identifying mixed species in processed foods. The first research proposing the use of next-generation sequencing (NGS) for identification of fish for food consumption appeared in 2012. Recently, several platforms used for NGS have shown their capacity to identify up to 15 different fish species or more in a single highly processed fish product.