Ultrasound-assisted aqueous two-phase extraction of resveratrol from the enzymatic hydrolysates of Polygonum cuspidatum

• Published in: Food bioscience Vol. 31; p. 100442
• Main Authors: Zhou, Linfang, Jiang, Bo, Zhang, Tao, Li, Shuhua
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2019
• Subjects: Hu zhang; Polydatin; Polydatin-β-glucosidase; Polygonum cuspidatum; Resveratrol; Polydatin-β-glucosidase; Hu zhang; Polygonum cuspidatum; Resveratrol; Polydatin
• ISSN: 2212-4292; 2212-4306
• DOI: 10.1016/j.fbio.2019.100442

An efficient ultrasound-assisted aqueous two-phase extraction (UAATPE) and enrichment process for resveratrol from the enzymatic hydrolysates of Polygonum cuspidatum using an ethanol/ammonium sulfate system was developed. After enzymatic hydrolysis using polydatin-β-glucosidase (PBG) which was a wild extracellular enzyme, 99.7% of polydatin was converted to resveratrol. The maximum yield of resveratrol was 10.7 mg/g and the corresponding recovery rate was 99.1% at 21% (w/w) ethanol/22% (w/w) (NH4)2SO4. The extraction yield of resveratrol in UAATPE was increased by 43.6 and 79.4% compared with ultrasound-assisted extraction and solvent extraction with stirring, respectively. Meanwhile, the total sugars in UAATPE were only 46.8% of ultrasound-assisted extraction and 56.0% of solvent extraction with stirring. The process of UAATPE is a promising technique for extraction and enrichment of bioactive components. •Using polydatin-β-glucosidase, 99.7% of polydatin was converted to resveratrol.•Ultrasound-assisted aqueous two-phase extraction (UAATPE) of resveratrol was done.•The aqueous two-phase systems of 21% (w/w) ethanol/22% (w/w) (NH4)2SO4 was optimal.•UAATPE is a promising technique for extraction and enrichment of bioactive components.