Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report

There is currently no "gold-standard" method to diagnose prosthetic joint infections (PJI), and the current practice of using microbiological cultures has many limitations. The identification of the bacterial species causing the infection is crucial to guide treatment; therefore, a robust...

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Bibliographic Details
Published inCurēus (Palo Alto, CA) Vol. 15; no. 5; p. e38788
Main Authors Wilkinson, Hollie, McCarthy, Helen S, Perry, Jade, Smith, Tony, Wright, Karina, Cool, Paul
Format Journal Article
LanguageEnglish
Published United States Cureus Inc 09.05.2023
Cureus
Subjects
Antibiotics
Bacteria
Biochemistry
Case reports
Classification
Debridement
E coli
Genetics
Infections
Infectious Disease
Medical diagnosis
Orthopedics
Prostheses
Transplants & implants
diagnosis
genomics
infection
orthopaedics
nanopore
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Summary:There is currently no "gold-standard" method to diagnose prosthetic joint infections (PJI), and the current practice of using microbiological cultures has many limitations. The identification of the bacterial species causing the infection is crucial to guide treatment; therefore, a robust method needs to be developed. Here, we attempt to use genomic sequencing with the MinION device from Oxford Nanopore Technologies to identify the species of bacteria causing PJI in a 61-year-old male. Genomic sequencing with the MinION presents an opportunity to produce species identification in real-time and at a smaller cost than current methods. By comparing results with standard hospital microbiological cultures, this study suggests that nanopore sequencing using the MinION could be a faster and more sensitive method to diagnose PJI than microbiological cultures.
Bibliography:ObjectType-Case Study-2
SourceType-Scholarly Journals-1
content type line 23
ObjectType-Report-1
ISSN:2168-8184
2168-8184
DOI:10.7759/cureus.38788