Selective turn-on near-infrared fluorescence probe for hypoxic tumor cell imaging

Hypoxia is a typical feature of solid tumors. To detect hypoxic tumor cells, we designed new selective turn-on probes by conjugating (1-methyl-2-nitro-1 H -imidazol-5-yl)methanol to the near-infrared fluorescence probe DCPO through an ether linkage and bis-carbamate linkage specifically. The propose...

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Bibliographic Details
Published inRSC advances Vol. 7; no. 30; pp. 18217 - 18223
Main Authors Jin, Chen, Zhang, Qiumeng, Lu, Wei
Format Journal Article
LanguageEnglish
Published 01.01.2017
Subjects
Biotechnology
Cytochromes P450
Ethers
Fluorescence
Hypoxia
Linkages
Selectivity
Tumors
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Summary:Hypoxia is a typical feature of solid tumors. To detect hypoxic tumor cells, we designed new selective turn-on probes by conjugating (1-methyl-2-nitro-1 H -imidazol-5-yl)methanol to the near-infrared fluorescence probe DCPO through an ether linkage and bis-carbamate linkage specifically. The proposed activation mechanism was demonstrated through the nitroreductase and cytochrome P450 assays in vitro . However, two probes revealed different hypoxia selectivities when evaluated on H460, HeLa and A549 cell lines. The hypoxia selectivity of IOD was higher than that of IND . The probe IOD (containing the ether linkage) was considered to be a promising hypoxia-selective fluorescent probe.
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ISSN:2046-2069
2046-2069
DOI:10.1039/C7RA01466J