Behavior of intracellular lipid droplets during cell division in HuH7 hepatoma cells

• Published in: Experimental cell research Vol. 433; no. 2; p. 113855
• Main Authors: Makiyama, Tomohiko, Obama, Takashi, Watanabe, Yuichi, Chatani, Masahiro, Azetsu, Yuki, Kawaguchi, Kosuke, Imanaka, Tsuneo, Itabe, Hiroyuki
• Format: Journal Article
• Language: English
• Published: United States 15.12.2023
• Subjects: Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Humans; Lipid Droplets - metabolism; Lipid Metabolism; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Mitosis; NEK2A; Perilipin; Triacylglycerol; Lipid droplets; Mitosis; Cell cycle
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2023.113855

Intracellular lipid droplets (LDs) are ubiquitous organelles found in many cell types. During mitosis, membranous organelles, including mitochondria, are divided into small pieces and transferred to daughter cells; however, the process of LD transfer to daughter cells is not fully elucidated. Herein, we investigated the behavior of LDs during mitosis in HuH7 human hepatoma cells. While fragments of the Golgi apparatus were scattered in the cytosol during mitosis, intracellular LDs retained their size and spherical morphology as they translocated to the two daughter cells. LDs were initially distributed throughout the cell during prophase but positioned outside the spindle in metaphase, aligning at the far sides of the centrioles. A similar distribution of LDs during mitosis was observed in another hepatocarcinoma HepG2 cells. When the spindle was disrupted by nocodazole treatment or never in mitosis gene A-related kinase 2A knockdown, LDs were localized in the area outside the chromosomes, suggesting that spindle formation is not necessary for LD localization at metaphase. The amount of major LD protein perilipin 2 reduced while LDs were enriched in perilipin 3 during mitosis, indicating the potential alteration of LD protein composition. Conclusively, the behavior of LDs during mitosis is distinct from that of other organelles in hepatocytes.