The Role of Hydroxylation of Proline in the Antigenicity of Basement Membrane Collagen

Rabbit antibodies to bovine basement membrane collagen were used to compare the antigenic determinants of rat parietal yolk sac basement membrane [14C]procollagen with [14C]protocollagen. Basement membrane [14C]protocollagen was found to be less antigenic than basement membrane [14C]procollagen. Hyd...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 117; no. 6; pp. 2181 - 2184
Main Authors Arbogast, Bradley W, Gunson, Diane E, Kefalides, Nicholas A
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.12.1976
Subjects
Animals
Basement Membrane - immunology
Cattle
Collagen - immunology
Epitopes
Hydroxylation
Hydroxylysine - pharmacology
Procollagen - immunology
Proline - metabolism
Rabbits
Radioimmunoassay
Rats
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Summary:Rabbit antibodies to bovine basement membrane collagen were used to compare the antigenic determinants of rat parietal yolk sac basement membrane [14C]procollagen with [14C]protocollagen. Basement membrane [14C]protocollagen was found to be less antigenic than basement membrane [14C]procollagen. Hydroxylation of basement membrane [14C]protocollagen, either intracellularly or in vitro with protocollagen prolyl hydroxylase, resulted in restoration of antigenicity. The difference in antigenicity observed between basement membrane [14C]procollagen and basement membrane [14C]protocollagen appeared to depend primarily upon the presence of hydroxyproline in the collagen molecule. Glucosylgalactosylhydroxylysine was found to be unimportant for antigenicity.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.117.6.2181