Validation of the AccuPoint Advanced ATP Hygiene Monitoring System for Sanitation Monitoring Through Detection of ATP from Stainless Steel Surfaces

• Published in: Journal of AOAC International Vol. 100; no. 2; pp. 537 - 547
• Main Authors: Viator, Ryan, Gray, R Lucas, Sarver, Ron, Steiner, Brent, Mozola, Mark, Rice, Jennifer
• Format: Journal Article
• Language: English
• Published: England 01.03.2017
• Subjects: Adenosine Triphosphate - analysis; Benzalkonium Compounds - chemistry; Decanoic Acids - chemistry; Fatty Acids - chemistry; Hygiene; Limit of Detection; Luciferases - chemistry; Luminescent Measurements; Pseudomonas aeruginosa - isolation & purification; Saccharomyces cerevisiae - isolation & purification; Sanitation; Sodium Hypochlorite - chemistry; Stainless Steel - analysis; Staphylococcus aureus - isolation & purification
• ISSN: 1060-3271; 1944-7922
• DOI: 10.5740/jaoacint.16-0311

The AccuPoint Advanced ATP Hygeine Monitoring System was validated by an AOAC International Performance Tested MethodSM on the detection of ATP from stainless steel surfaces. Neogen Corp.'s system is a lightweight, hand-held diagnostic tool used to validate and verify a hygiene program's effectiveness by detecting organic residues remaining on surfaces and in liquids after cleaning. The system is composed of three primary components: an electronic luminometer, fully self-contained single-use samplers, and software. The system is designed to detect adenosine triphosphate (ATP) at set thresholds and to report the measurement in relative light units (RLU). These thresholds are established by a facility to reflect effective cleaning practices. The instrument compares the measured level of ATP with the established threshold and reports the results as pass, marginal, or fail. A linear dose-response in RLU was observed with pure analyte. In the matrix and microbial studies, detection levels varied depending on the matrix and microorganism tested. Independent laboratory trials confirmed pure analyte and matrix observations. Specificity testing of similar, yet different, compounds resulted in 0 RLU for all except 2'-deoxyadenosine 5'-triphosphate sodium salt, which showed markedly reduced reactivity when compared with ATP. Also, interference by these compounds was negligible. When disinfectant residues were evaluated for their effect on the test, cleaners increased RLU output to varying degrees. Stability testing showed consistent results between three independently manufactured lots and stable results through the 9 month shelf-life. Additionally, when three readers were compared using electronic light-emitting diodes as the light source, instrument variability was low (<3%). Robustness testing results provided evidence that temperature affects test performance more than shaking time, and sampler performance improves as the temperature increases to room temperature. These results provided evidence that the AccuPoint Advanced ATP Hygiene Monitoring System produces consistent and reliable data for the evaluation of sanitation program effectiveness on stainless steel surfaces in food processing and food service facilities.