Transfer of the waterfall source isolate Pectobacterium carotovorum M022 to Pectobacterium fontis sp. nov., a deep-branching species within the genus Pectobacterium

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 2; pp. 470 - 475
• Main Authors: Oulghazi, Said, Cigna, Jérémy, Lau, Yin Yin, Moumni, Mohieddine, Chan, Kok Gan, Faure, Denis
• Format: Journal Article
• Language: English
• Published: England Microbiology Society 01.02.2019
• Subjects: Life Sciences; Dickeya; Pectobacterium; pectinolytic; water; waterfall; stem rot; bacterial soft-rot; subsp carotovorum; wasabiae; 1st report; taxonomy; potato; atrosepticum; brasiliense; pathogen
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003180

Pectobacterium carotovorum M022 has been isolated from a waterfall source in Selangor district (Malaysia). Using genomic and phenotypic tests, we re-examined the taxonomical position of this strain. Based on 14 concatenated housekeeping genes (fusA, rpoD, rpoS, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA and rplB), multi-locus sequence analysis revealed that strain M022 falls into a novel clade separated from the other Pectobacterium species. The in silico DNA-DNA hybridization and average nucleotide identity values were lower than the 70 and 95 % threshold values, respectively. In addition, by combining genomic and phenotypic tests, strain M022 may be distinguished from the other Pectobacterium isolates by its incapacity to grow on d(+)-xylose, l-rhamnose, cellobiose and lactose. Strain M022 (=CFBP 8629 =LMG 30744 ) is proposed as the type strain of the Pectobacteriumfontis sp. nov.