Osteoglycin Expression Influences Gelatinases Activity of Murine Hepatocarcinoma Cells Cultured with Extract of Lymph Node
Objective: To investigate the possible correlation between osteoglycin expression and gelatinases activity of tumor cells. Methods: Eukaryotic expression plasmid and antisense expression plasmid of osteoglycin were constructed, and transfected into mouse hepatocarcinoma Hca-F cells and Hca-P cells,...
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Published in | Chinese journal of cancer research Vol. 21; no. 3; pp. 171 - 176 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
Chinese Anti-Cancer Association
01.09.2009
Department of Oncology, the First Affiliate Hospital, Dalian Medical University, Dalian 116011, China%Department of Pathology, Dalian Medical University, Dalian 116044, China |
Subjects | |
Online Access | Get full text |
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Summary: | Objective: To investigate the possible correlation between osteoglycin expression and gelatinases activity of tumor cells.
Methods: Eukaryotic expression plasmid and antisense expression plasmid of osteoglycin were constructed, and transfected into mouse hepatocarcinoma Hca-F cells and Hca-P cells, respectively. RT-PCR and Western blot were employed to analyze osteoglycin expression in tumor cells. Zymographic analysis was used to observe the possible correlation between osteoglycin expression and gelatinases activity of tumor cells cultured under different conditions.
Results: High expression of osteoglycin after transfection of osteoglycin gene attenuated secretion of gelatinases in Hca-F cells cultured with extract of lymph node (P〈0.05). However, transfection of osteoglycin gene into Hca-F cells failed to influence gelatinases activity of tumor cells cultured with extracts of liver and spleen or in DMEM medium. Effective suppression of osteoglycin expression in Hca-P cells by osteoglycin shRNA resulted in enhanced activity of gelatinases in Hca-P cells cultured with extract of lymph node (P〈0.05). However, inhibition of osteoglycin expression in Hca-P cells failed to influence gelatinases activity of tumor cells cultured with extracts of liver and spleen or in DMEM medium.
Conclusion: Osteoglycin expression influences gelatinases activity of murine hepatocarcinoma cells cultured with extract of lymph node. Regulation of gelatinases activity might be one of the mechanisms by which osteoglycin contribute to lymphatic metastasis suppression. |
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Bibliography: | Osteoglycin; Transfection; shRNA; Hepatocellular carcinoma; Metastasis; Gelatinases Transfection Osteoglycin shRNA Gelatinases Q813.11 11-2591/R Hepatocellular carcinoma TQ925 Metastasis |
ISSN: | 1000-9604 1993-0631 |
DOI: | 10.1007/s11670-009-0171-z |