Probing with Synthetic Peptides the Interactive Properties of N-Butylphenyl and Cholesterol-10-Undecenaoate Phases Immobilised onto Open Tubular Electrochromatographic Capillaries

• Published in: Chromatographia Vol. 61; no. 7-8; pp. 351 - 357
• Main Authors: Matyska, M. T, Pesek, J. J, Chen, J. I-Chen, Boysen, R. I, Hearn, M. T. W
• Format: Journal Article
• Language: English
• Published: Oxford Vieweg Verlag 01.04.2005; Springer
• Subjects: Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; chromatography; electrolytes; electrophoresis; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; hydrophobicity; ligands; migratory behavior; Other chromatographic methods; Proteins; solvents; synthetic peptides; Capillary column; Coating material; Phase composition; Peptides; Electrochromatography Proteomic analysis Modulation of selectivity Resolution optimisation Synthetic peptides; Immobilization; Primary structure; Selectivity; Mobile phase; Optimization; Ultraviolet detector; Synthetic product; Electrophoretic mobility; Electrochromatography; Proteomics; pH; Property structure relationship; Modified material
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-004-0477-4

This study investigates the impact of peptide primary structure and the characteristics of two different types of immobilised hydrophobic ligands on the open tubular electro-chromatographic migration behaviour of a family of related synthetic peptide analogues. The results further document the important role that mixed mode electrophoretic/chromatographic processes play in determining peptide selectivity in OTCEC systems. Procedures to fine-tune selectivity of closely related peptides in these systems have been established with buffer electrolytes of different composition in terms of the organic solvent content and different pH conditions.