Montmorillonite adsorbs creatinine and accelerates creatinine excretion from the intestine

• Published in: Journal of pharmacy and pharmacology Vol. 61; no. 4; p. 459
• Main Authors: Zhang, Yan-Ting, Wang, Xiu-Fang, Long, Li-Hui, Liu, Tong, Cao, Yong-Xiao
• Format: Journal Article
• Language: English
• Published: England 01.04.2009
• Subjects: Adsorption; Animals; Bentonite - administration & dosage; Bentonite - pharmacology; Creatinine - administration & dosage; Creatinine - blood; Creatinine - pharmacokinetics; Hydrogen-Ion Concentration; Intestinal Absorption - drug effects; Mice; Mice, Inbred ICR; Rats; Rats, Sprague-Dawley; Time Factors
• ISSN: 0022-3573
• DOI: 10.1211/jpp.61.04.0007

This study aims to evaluate the sorption by montmorillonite of creatinine and the accelerating effect of montmorillonite on creatinine excretion from the intestine. The sorption of montmorillonite was observed in vitro. Also, rat intestinal tract and blood vessels were perfused circularly with perfusate with or without creatinine, respectively, to study the promotion of creatinine diffusion from the blood vessel to the intestine and the inhibition of creatinine absorption in the intestinal tract. The effect of decreasing the serum concentration of creatinine was studied in an acute hypercreatininaemia mouse model. The concentration of creatinine was determined by the basic picric acid method. Montmorillonite adsorbed creatinine markedly in the simulated intestinal solution in a concentration-dependent manner. The sorption-time curve of montmorillonite with creatinine showed that the sorption was fast. The adsorption rate reached a maximum in 10 min. The pH of the solution influenced the sorption, the rate of which was higher at a low pH than at a high pH. Creatinine could diffuse from the blood vessel to the intestine and was reabsorbed in the intestine. Montmorillonite promoted the diffusion and inhibited the absorption. Montmorillonite decreased the serum creatinine level of hypercreatininaemia mice prepared by injecting creatinine intraperitoneally. Montmorillonite adsorbs creatinine and accelerates its excretion from the intestine.