Human papillomavirus (HPV) cervical lesions: results from 300 Italian women studied with DNA hybridization techniques and morphology

Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HP...

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Bibliographic Details
Published inTumori Vol. 74; no. 6; p. 745
Main Authors Nuzzo, F, Tison, V, Castagnoli, A, Tiboni, M, De Villiers, E M
Format Journal Article
LanguageEnglish
Published United States 31.12.1988
Subjects
Cervix Uteri - microbiology
Cytodiagnosis
DNA, Viral - analysis
Female
Humans
Nucleic Acid Hybridization
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
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Summary:Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HPV-DNA in cervical biopsy specimens). The prevalence of HPV cervical infection diagnosed by PAP smears was 11.6%. Despite disadvantages, filter in situ hybridization was confirmed to be particularly useful for screening purposes to detect HPV in cervical scrapings. In 3 cases it was the only applicable method for diagnosing "high-risk" HPV infection. Southern blot hybridization of tissue DNA with HPV 16-DNA revealed the presence of this virus in 8 cases, and HPV 31-DNA and HPV 42-DNA in 1 case each.
ISSN:0300-8916
DOI:10.1177/030089168807400621