Effect of consumption of anthocyanin-rich products on NMR lipoprotein subclasses and biomarkers in hypercholesterolemic subjects: a randomized controlled trial (the AppleCOR study)

• Published in: Food & function Vol. 16; no. 6; pp. 2279 - 229
• Main Authors: Pedret, A, Llauradó, E, Calderón-Pérez, L, Companys, J, Pla-Pagà, L, Salamanca, P, Sandoval-Ramírez, B. A, Besora-Moreno, M, Catalán, Ú, Fernández-Castillejo, S, Ludwig, I, Macià, A, Rubió-Piqué, L, Sampson, M, Remaley, A. T, Valls, R. M, Motilva, M. J, Solà, R
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 17.03.2025
• Subjects: Adult; Aged; Alanine; Anthocyanins; Anthocyanins - administration & dosage; Anthocyanins - metabolism; Apples; Biomarkers; Biomarkers - blood; Female; Fruit - chemistry; Fruit - metabolism; High density lipoprotein; Humans; Hypercholesterolemia - blood; Hypercholesterolemia - diet therapy; Hypercholesterolemia - metabolism; Lipoproteins - blood; Lipoproteins - metabolism; Low density lipoprotein; Magnetic Resonance Spectroscopy; Male; Malus - chemistry; Malus - metabolism; Metabolites; Middle Aged; NMR; Nuclear magnetic resonance
• ISSN: 2042-6496; 2042-650X; 2042-650X
• DOI: 10.1039/d4fo02949f

Our aim was to assess the effect of intake of anthocyanin biofortified red-fleshed apples (RFA) versus that of common white apples (WFA) without anthocyanins on the NMR lipoprotein subfraction profile and other NMR metabolites. Additionally, an aronia infusion (AI) arm, matching the anthocyanin content and profile of the RFA, was included. A 6-week, randomized, parallel study was conducted in hypercholesterolemic subjects ( n = 121). Anthocyanin-rich products (RFA and AI) decreased LDLc; ApoB; total, large, and small LDL-P; LDL size; TG/HDL ratio; and large TRL, versus WFA. All treatments significantly decreased HDLc, ApoA1, and total HDL-P, with the most significant reductions after RFA treatment. RFA significantly decreased large HDL-P compared to WFA and AI, while medium HDL-P decreased significantly after AI compared to WFA. Anthocyanin-rich products decreased GlycA and alanine and increased acetoacetate versus WFA. WFA and RFA decreased plasma citrate versus AI. Thus, anthocyanin-rich products provided greater protection against CVD risk than WFA. Anthocyanin-rich products (RFA and AI) decreased LDLc; ApoB; total, large, and small LDL-P; LDL size; TG/HDL ratio, large TRL, and GlycA and alanine versus WFA. Thus, anthocyanin-rich products provided greater protection against CVD risk than WFA.